CpG ODN 对卵清蛋白致敏的哮喘小鼠模型的免疫调节作用研究

Effect of CpG oligodeoxynucleotides in OVA antigen induce allergic airway reaction in mice

关键词:  CpG ODN  哮喘； 免疫调节

Key words:  CpG oligodeoxynucleotides  asthma  immunotherapy

摘要:

［目的］ 研究寡核苷酸免疫刺激序列CpG ODN（CpG Oligodeoxyneucleotide）对卵清蛋白(OVA)致敏哮喘小鼠的免疫调节作用，探讨哮喘的非激素治疗方法。［方法］以OVA免疫BALB/c小鼠，致敏前和致敏阶段腹腔注射CpG ODN,分别用地塞米松和生理盐水作为激素治疗组和哮喘对照组。 流式细胞仪检测各组哮喘小鼠的外周血T淋巴细胞亚群；ELISA法检测各组小鼠血清IL-4,IFN-γ，Ig-E水平；HE染色观察各组哮喘小鼠肺组织病理变化。［结果］ 与哮喘对照组相比，CpG组能明显降低血清IL-4水平，NS组：（106.97±13.40） pg/mL, CpG组: （87.69±13.25） pg/mL；降低血清Ig-E水平，NS组：（4.78±1.65） IU/mL，CpG组：（3.52±0.23）IU/mL（P<0.05）；增加血清IFN-γ含量，NS组: （34.76±1.51）pg/mL, CpG组: （49.93±9.18）pg/mL(P<0.05)；上调CD4/CD8的比值，NS组：（3.53±0.37），CpG组：（3.73±0.55)（P<0.05 ）；CpG组和地塞米松组均能抑制肺组织中嗜酸细胞的浸润（P<0.05）。［结论］ CpG ODN能有效促进哮喘小鼠体内TH2反应向TH1反应，可作为哮喘的预防和治疗的非激素手段。

Abstract:

［Objective］ To investigate the effect of CpG-motif oligodeoxy nucleotides (CpG ODN) on the antigen （OVA） induced allergic airway reaction in mice. ［Methods］ The asthma model was set up in the BALB/c mice with OVA, Before the antigen insensitization stage, CpG ODN、Anaflogistico and NS were used intra peritoneal. During the state of antigen insensitization, three groups did the same processing. ［Results］ Compared with the NS control, coadministration of CpG ODN insensitization phase significant reduced the serologic concentrations of IL-4, NS group：(106.97±13.40) pg/mL, CpG group: (87.69±13.25) pg/mL;reduce the serologic concentrations of Ig-E, NS group：(4.78±1.65) IU/mL，CpG group：(3.52±0.23) IU/mL（P<0.05）and increased of IFN-γ level in serum, NS group: (34.76±1.51) pg/mL, CpG group: (49.93±9.18) pg/mL （P<0.05）. CpG ODN group updated the serologic CD4/CD8 ratio from (3.53±0.37) to (3.73±0.55)（P<0.05）. Coadministration of CpG ODN also reduced the percent of EOS in lung tissue (P<0.05). ［Conclusion］ This study suggests that in-traperitoneal coadministration of CpG ODN enhances Th1 cytokine such as IL-4 and Ig-E expression, down-regulates Th2 cytokine (IFN-γ) expression. It plays an important role of immunomodulation on Th1/Th2. This study also suggests that CpG ODN immunization can be an effective non-hormone therapy for asthma.