乌苏里藜芦碱在大鼠的血液流变学作用

Haemorheological effect of veratrum nigrum L.Var.Ussurience Nakai alkaloids in rats

关键词:  乌苏里藜芦碱  血液流变学  血液粘度

Key words:  :veratrum nigrum L.Var.ussurience Nakai alkaloids  haemorrheology  blood viscosity

摘要:

研究乌苏里藜芦碱（VnA）对大鼠血液粘度的影响。［方法］ 正常和血淤大鼠iv VnA 15 μg·kg-1,30μg·kg-1和45μg·kg-1后取血，以微机化血液比粘度仪测定全血比粘度、血浆比粘度和红细 胞压积等血液流变学指标。［结果］ 正常大鼠iv VnA后全血比粘度显著降低,低切变率时自20.87±3.63(NS组)降至13.08±2.42(VnA中剂量组， P<0.01)和11.89±4.77（VnA大剂量组，P<0.01），高切变率时自6.14±1.05（NS组）降至4.71±1.03（VnA大剂量组，P<0.05）。血淤模型大鼠iv VnA同样导致全血比粘度的显著降低，在低切变率时中剂量组为18.98±3.36，大剂量组为8.35±3.34,而血淤模型组为50.81±7.54, P<0.01，在高切变率时，大剂量组为8.32±2.74，而血淤模型组为12.57±3.06，P<0.05。VnA在大剂量时对血浆粘度也有一定降低作用（1.46±0.03 vs 1.53±0.06，P<0.05,正常大鼠；1.57±0.12 vs 1.87±0.44，P<0.01,血淤大鼠）。对血细胞压积无显著影响。［结论］ 静脉给予VnA对血粘度具有明显的降低作用，对低切变率全血粘度的降低尤为明显。其降粘作用不是红细胞数量和体积改变的结

Abstract:

Objective］ To investigate the effect of veratrum nigrum L .Var. ussurience Nakai alkaloids （VnA）on rat blood viscosity. ［Methods］ Normal and blood stasis model rats were administered intravenously VnA at doses of 15,30 and 45μg·kg-1.The blood samples were collected and tested for haemorheological parameters including whole blood viscosity(WBV), plasma viscosity and HCT by means of computer-aided viscometer.［Results］ In normal rats the WBV at low shear rate(SR) was significantly reduced by iv VnA from 20.87±9.63(NS group) to 13.08±2.42 (middle dose group,P<0.01) and 11.89±4.77(large dose group,P<0.01),at high SR from 6.14±1.05(NS group) to 4.71±1.03(large dose group, P<0.05 ).In blood stasis model rats, WBV was markedly reduced by iv administration at low SR (18.98±3.36 for middle dose and 8.35±3.34 for large dose vs 50.81±7.54 for blood stasis model) and at high SR(8.32±2.74 for large dose vs 12.57±3.06 for blood stasis model,P<0.05).It was found that VnA at large dose had some lowering effect on plasma viscosity (1.46±0.03 vs 1.53±0.06 and 1.57±0.12 vs 1.87±0.44 in normal and blood stasis model,respectively, P<0.05 and P<0.01),and no significant effect on HCT could be measured following administration of VnA. ［Conclusion］ VnA administered by iv has significant lowering effect on blood viscosity ,especially, on WBV at low SR,which is independent on number and volume of RBC.