缺血再灌注损伤降低肠黏膜屏障功能的初步研究

Decrease of intestinal barrier after ischemia-reperfusion injury in animals

关键词:  缺血再灌注  肠黏膜屏障  细菌易位

Key words:  ischemia-reperfusion  intestinal barrier  bacterial translocation

摘要:

观察缺血再灌注损伤对肠黏膜屏障功 能的影响。［方法］ 将10只昆明种小鼠和16 只SD大鼠随机分为对照组和缺血再灌注组，小鼠每组5只，大鼠每组8只。取小鼠空肠和结肠 肠壁组织，制备组织切片，观察光镜及电镜下肠黏膜形态学变化；取大鼠肠系膜前淋巴结， 细菌培养48 h后观察细菌易位变化。［结果］ 光镜下观察到缺血再灌组空肠肠壁变薄，绒毛短细，绒毛间距变宽，局部黏膜上皮坏死脱落，固有层充血淤血，结肠变化不明显；电镜下 缺血再灌组与对照组相比未见明显改变；缺血再灌组细菌易位率为5/8，对照组为1/8（P <0.05）。［结论］ 缺血再灌注对小鼠和大鼠肠黏膜机械和免疫屏障有一定程度的损伤。

Abstract:

To observe the changes of intestinal b arrier after ischemi a-reperfusion injury (I/R) in mice and rats. ［Methods］ 10 mice of Kunming spec ie s and 16 rats of SD species were divided randomly into 2 groups: control group a nd I/R group. There are 5 mice and 8 rats in each group. The mice's jejumum and colon were exenterated and made into slides, and their morphological changes wer e observed under light and electrical microscopes. The bacterial translocation r ates of mesenteric lymph nods(MLN) were detected after 48 hours culture of rats' MLN. ［Results］ Compared to control group, obvious morphological changes were o bserved in jejunum, but not in colon, under LM in I/R group: the intestinal wall became thinner, the intestinal villi grew shorter and the interspace between the villi became wider, and parts of the intestinal villi underwent necrosis and b roke off, also there were congestion and hemorrhage in lamina propria. No distinct changes of tight junction were observed under EM in jejunum and colon after I /R. The bacterial translocation rates of MLN is 5/8 in I/R group and 1/8 in cont rol group（P<0.05）. ［Conclusion］ Ischemia-reperfusion injury induces inte stinal mucosal damage and bacterial translocation in mice and rats.