血红蛋白、转铁蛋白联合免疫法检测便潜血在临床中的应用

United immunoassay that used for clinic in test of dung occult blood

关键词:  便潜血  单克隆抗体法  化学法  免疫法

Key words:  occult blood  monoclonal antibody  united immunoassay  benzidine

摘要:

评价血红蛋白、转铁蛋白联合免疫法（联合免疫法）检测便潜血的临床应用价值。［方法］①比较联合免疫法、单克隆抗体法与联苯胺法对5种质量浓度人血红蛋白液及500μg/mL的4种动物血红蛋白液检测的灵敏度差异。②用联合免疫法检测6种不同质量浓度的转铁蛋白液以确定检出最低限值。③比较联合免疫法、单克隆抗体法检测44例消化道疾病患者便潜血的阳性检出率；其中包括4例胃肿瘤性病变，25例上消化道出血，8例溃疡性结肠炎及7例大肠肿瘤性病变。［结果］①联合免疫法、单克隆抗体法及联苯胺法最低检出限量分别为0.2、0.2及100 μg/mL；②联苯胺法对人和动物的血红蛋白均发生反应，单克隆抗体法只与人的血红蛋白反应，而联合免疫法只对人的血红蛋白、转铁蛋白发生抗原抗体反应，从而进一步提高了单克隆抗体法的阳性检出率。对于4例胃肿瘤性病变、25例上消化道出血、8例溃疡性结肠炎及7例大肠肿瘤性病变，单克隆抗体法分别检出3例、17例、8例、6例，联合免疫法分别检出4例、21例、8例、7例。［结论］联合免疫法灵敏度高、特异性强。因可同时测定血红蛋白和转铁蛋白，可提高消化道出血及大肠肿瘤的阳性检出率

Abstract:

To evaluate the clinical vaule of the united immunoassay being to detect dung occult blood.［Methods］ ①To compare the sensitive difference among united immunoassay, monoclonal antibody and benzidine by testing hemoglobin from human beings and four different kinds of animals; ②The sensitivity of united immunoassay was used to detect transferrin(TF) with six different concentrations; ③ United immunoassay and monoclonal antibody were used to detect dung occult blood from 44 patients that were diagnosed as disease of alimentary canal including 4 with stomach cancer, 25 hemorrhage of upper digestive tractis, 8 colitis and 7 cancer of large intestine and then compare their positive rate of two methods. ［Results］ ① Sensitivities of united immunoassay, monoclonal antibody and benzidine to detect hemoglobin were 0.2 μg/mL，0.2 μg/mL and 100 μg/mL respectively. ②The monoclonal antibody can only detect human beings' hemoglobin, while the united immunoassay can detect human beings' hemoglobin and TF, but benzidine can detect those from both human and animals. The detected rates in 4 stomach cancer, 25 hemorrhage of upper digestive tractis, 8 colitis and 7 cancer of large intestine, those were 3,17,8 and 6 monoclonal antibody with united immunoassay were 4, 21, 8 and 7 and with respectively.［Conclusion］ The united immunoassay has high sensitivity.