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| 噬菌体肽库中筛选NMDA受体模拟抗原 |
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康晓楠1, 孙长凯1, 范明2, 王芳2, 丁爱石2, 施广霞3
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1.大连医科大学,脑疾病研究所,辽宁,大连,116027;2.军事医学科学院基础医学研究所;3.大连医科大学,病理生理学教研室,辽宁,大连,116027
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| 摘要: |
| [目的] 研究发现脑缺血患者体内存在N甲基-D-门冬氨酸(NMDA)受体自身抗体,了解其滴度与疾病程度的相关性.[方法] 用NMDAR1单克隆抗体淘筛噬菌体展示随机12肽库,获得NMDAR1模拟表位抗原.[结果] 细胞竞争ELISA结果显示,该模拟抗原可以竞争细胞表面天然抗原与抗体的结合,具有抗原模拟性.[结论] 噬菌体肽库中筛选NMDAR1受体模拟抗原可以用于临床病人血清检测.本研究为下一步临床检验准备了必要条件. |
| 关键词: 噬菌体展示 自身抗体 模拟表位 NMDA受体 |
| DOI：10.11724/jdmu.2002.03.01 |
| 分类号:Q5 |
| 基金项目:国家自然科学基金，中国博士后科学基金，辽宁省科技攻关项目，军事医学科学院基础医学研究所科研项目 |
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| To screen the minotope of NMDAR1 from phage display peptide library |
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KANG Xiao-nan1, SUN Chang-kai1, FAN Ming2, WANG Fang2, DING Ai-shi2, SHI Guang-xia3
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1.Institute of Brain Disease, Dalian Medical University, Dalian 116027, China;2.Basic Medicine Institute, Military Medical Academy,Beijing 100850,China;3.Departement of Pathophysiology, Dalian Medical University, Dalian 116027, China )
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| Abstract: |
| To determine the minotope of NMDAR1.［Methods］The monoclonal antibody against the NMDAR1 was used as target protein to screen the binding peptide from a 12-mer Ph.D. random peptide library. After three rounds of affinity screening，the peptide sequences of positive phage clones were determined and analyzed by DNA sequencing，ELISA and competitive inhibition. ［Results］ A positve clone was found. It could competitively inhibit the native antigen binding to mAbN1. ［Conclusion］ This peptide mimics native NMDAR1 and may be useful for NMDAR1 autoantibody diagnosis in the patients with chronic cerebral circulation disorder. |
| Key words: phage display autoantibody minotope NMDAR |