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| P-170糖蛋白在多药耐药(MDR)K562/ADM细胞中的定位研究 |
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高船舟1, 曲淑贤1, 吕广艳1, 郝立宏2, 杨佩满2
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1.大连医科大学分析测试中心,辽宁,大连,116027;2.大连医科大学组织胚胎学教研室,辽宁,大连,116027
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| 摘要: |
| 确定P-170糖蛋白在多药耐药(MDR)K562/ADM细胞中表达的位置。方法：通过免疫反应并利用流式细胞仪(FCM)检测表达P-170糖蛋白细胞的百分含量；用免疫电镜技术检测P-170糖蛋白在细胞中的位置及其表达水平。结果：敏感株K562细胞与耐药株K562/ADM细胞对P-170糖蛋白的表达有非常明显的差异；不同的K562/ADM细胞表达P-170糖蛋白的量差别较大；少量的K562细胞也有极少的P-170糖蛋白的表达；细胞表达的P-170糖蛋白位于细胞膜上。结论：多药耐药K562/ADM细胞表达的P-170糖蛋白最终被镶嵌在细胞膜上；在该蛋白质从被表达、修饰和转运到细胞膜上的过程中，该蛋白质不具有成熟P-170糖蛋白的特异构象 |
| 关键词: 多药耐药 P-170糖蛋白 免疫电镜 |
| DOI：10.11724/jdmu.2002.02.04 |
| 分类号:R73-3 |
| 基金项目: |
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| Confirming the position of P-glycoprotein in K562/ADM cells by the method of immune electron microscope |
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GAO Chuan-zhou,QU Shu-xian,L Guang-yan,HAO Li-hong
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Central of Analysis and Test, Dalian Medical University, Dalian 116027, China
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| Abstract: |
| To confirm the position of P-glycoprotein in K562/ADM cells with immune electron microscope(IEM) technology. Method: The percent of K562/ADM cells of expressing P-glycoprotein was mensurated by Flow cytometer (FCM); The position and expressing level of P-glycoprotein in K562/ADM cells were confirmed with IEM. Results: There are obvious differentiation of expressing level of P-glycoprotein in K562 and K562/ADM cells, K562/ADM cell line is higher than K562. Every cell of K562/ADM expresses P-glycoprotein with different quantity, but a few of K562 cells expressed a little P-glycoprotein. The P-glycoprotein is incrusted in cell membrane finally. Conclusions: P-glycoprotein is incrusted in cell membrane of K562 and K562/ADM cells. |
| Key words: multidrug resistance (MDR) P-glycoprotein immune electronic microscope |