慢性应激抑郁模型大鼠海马5-HT1A受体的变化

Change of 5-HT1A receptor in stress-induced depression rat hippocampus

关键词:  抑郁症  抗抑郁剂  应激  5-HT1A受体

Key words:  depression  antidepressants  stress  5-HT1AR

摘要:

探讨慢性不可预见性应激抑郁模型大鼠海马5-HT1A受体(5-HT1AR)的变化以及三环类抗抑郁剂(TCAs)阿米替林和5-羟色胺再摄取抑制剂(SSRI)氟西汀对其影响。方法：将24只SD雄性大鼠随机均分为4组，即对照组、抑郁组、阿米替林治疗组、氟西汀治疗组。应用［3H］8-OH-DPAT作为放射性配基，使用放射性配体受体结合法测定大鼠海马5-HT1AR的特异性结合。结果：与对照组相比，抑郁组大鼠海马 ［3H］8-OH-DPAT 特异性结合下降28.10%(P<0.05)。阿米替林治疗21 d，抑郁大鼠海马的［3H］8-OH-DPAT 特异性结合明显提高至(26.63±5.50) fmol/mg protein，与抑郁组(18.78±5.62) fmol/mg protein比较有显著性差异(P<0.05)，与对照组（26.12±5.52) fmol/mg protein比较无显著性差异（P>0.05）；氟西汀治疗21 d，抑郁大鼠海马的［3H］8-OH-DPAT特异性结合无显著变化（P>0.05）。结论：①抑郁大鼠海马5-HT1AR特异性结合下降可能与抑郁症病因相关。②海马 5-HT1AR可能是阿米替林发挥抗抑郁作用的环节。③氟西汀可能不通过影响海马突触后膜的5-HT1AR发挥抗抑郁作

Abstract:

To investigate the change of 5-HT1A receptor (5-HT1AR) from chronic unpredicted mild stress depression rat hippocampus and the effect of Amitriptyline and Fluoxetine on 5-HT1AR in former depression model. Methods: Male Sprague-Dawley rats were randomly allocated to four groups as follows: control, depression model, depression model treated by Amitriptyline and depression model treated by Fluoxetine. The radioligand binding that used ［3H］8-OH-DPAT as a radioactive ligand determines the special binding of 5-HT1AR. Results : The special binding of 5-HT1AR in model group decreased 28.10%,compared with control group (P<0.05). After Amitriptyline treatment the special binding of 5-HT1AR in model group obviously recover to the normal (P<0.05). The decreased 5-HT1AR was not effected by Fluoxetine (P>0.05). Conclusion: 1. It suggested that decreased 5-HT1AR in hippocampus may be associated with the etiology of depressive disorder. 2. The increased 5-HT1AR in hippocampus might be related to Amitriptyline's therapeutic effect in depressive disorder. 3. 5-HT1AR in hippocampus mightn't play a major role in the Fluoxetine's therapeutic effect of antidepressant treatment.