引用本文:邵淑娟,徐先发,张建军,杨佩满,程书钧,高燕宁.中国人喉鳞癌染色体9p21.3-23区域LOH分析[J].大连医科大学学报,2002,24(2):.
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中国人喉鳞癌染色体9p21.3-23区域LOH分析
邵淑娟1, 徐先发2, 张建军2, 杨佩满1, 程书钧2, 高燕宁2
1.大连医科大学组织胚胎学教研室,辽宁,大连,116027;2.中国医学科学院协和医科大学肿瘤医院肿瘤研究所病因研究室,北京,100021
摘要:
分析染色体9p21.3-23区域等位基因杂合性缺失(loss of heterozygosity,LOH)情况,确定其在中国人喉鳞癌中发生频率和最小缺失区域,为定位克隆喉鳞癌相关抑癌基因提供线索。方法: 应用9个微卫星多态性标记对24例喉鳞癌组织及其对应的正常远端肺组织进行了同位素标记的聚合酶链反应和变性凝胶电泳,并探讨各位点LOH与病理分级和病理分期的关系。结果: 24例喉鳞癌组织中,共有23例(95.83%)存在至少一个位点的LOH,丢失频率最高的位点位于9p22-23的D9S1782(100%)其次是位于9p22-23的D9S162(85.71%)。结论 : 在9p21.3-23区域可能存在两个或两个以上的与喉鳞癌发生相关的候选抑癌基因,分别位于9p22-23的D9S1782和D9S162附近。
关键词:  喉肿瘤  鳞状细胞癌  杂合性缺失  抑癌基因  定位克隆
DOI:10.11724/jdmu.2002.02.01
分类号:R739.65
基金项目:国家重点基础研究发展计划(973计划)
Loss of heterozygosity on chromosome 9p21.3-23 in laryngeal squamous cell carcinoma
SHAO Shu-juan1, XU Xian-fa2, ZHANG Jian-jun2, YANG Pei-man1, CHENG Shu-jun2, GAO Yan-ning2
1.Department of Histology and Embryology, Dalian Medical University,Dalian 116027 China;2.Cancer Institute (Hospital), Perking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021,China)
Abstract:
To determine the frequency and common deletion region of allelic losses on chromosome 9p21.3-23 in the laryngeal squamous cell carcinoma and facilitate the isolation of the candidate tumor suppressor genes associated with the development of laryngeal squamous cell carcinoma. Methods: Using 9 microsatellite markers, we mapped the region deleted frequently on 9p21.3-23 of tumor tissues of primary laryngeal squamous cell carcinoma surgically resected in 24 cases. We also analyzed the relationship between the loss of heterozygosity (LOH) on each locus and the pathological grade and stage. Results: Among 24 cases, 23 (95.83%) samples showed allelic loss of the 9p21.3-23 locus in at least one. The most frequent LOH loci were located in two regions: D9S1782 with in 7 of 7(100%) and D9S261 with in 6 of 7 (85.71%) locus on 9p21.3-23. Conclusion: These data have shown an interval of common deletion, encompassing D9S1827, D9S261 which might localize at least two candidate tumor suppressor genes in 9p21-23 region, which associated with occurence of laryngeal squamous cell carcinoma.
Key words:  squamous cell carcinoma  laryngeal neoplasms  tumor suppressor gene  LOH  position clone