| 摘要: |
| 目的:探讨肝癌细胞的膜磷脂组分变化.方法:采用高效薄板层析方法并配合无机磷的测定,比较了大鼠正常肝细胞与癌变细胞磷脂的组成及摩尔分数,用气相色谱法对其中含量最多的磷脂组分磷脂酰胆碱(PC)的脂肪酸组成进行了测定.结果与结论:肝癌细胞的PC含量明显减少(P<0.05),PC中长链及不饱和脂肪酸含量显著减少(P<0.05). |
| 关键词: 薄板层析 气相色谱 磷脂 磷脂酰胆碱 脂肪酸 |
| DOI：10.11724/jdmu.2001.04.03 |
| 分类号:O658 |
| 基金项目: |
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| Analysis of phospholipids and fatty acids composition of rat hepatoma cells |
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LI Ya-li1, MAN Hong-sheng2, XIA Quan1, MA Ke-li1, CUI Zhao-chun1
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1.Department of Biochemistry, Dalian Medical University, Dalian 116027, China;2.Center of Analysis and Test
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| Abstract: |
| To study the membrane phospholipid composition of rat hepatoma cells. Method: The mole fraction of phosphatidylcholine (PC) and phosphatidy lethanolamine (PE) in total phospholipids of CBRH-7919 hepatoma cells was compared to that of normal liver cells by high performance thin layer chromatography (HPTLC) and phosphorus assay. The composition and mole fraction of fatty acids of PC in hepatoma cells were also compared to those of normal ones with gas chromatographic analysis (GC analysis). Results and Conclusion: The PC content of membrane phospholipids in CBRH-7919 tumor cells, particularly in long chain and unsaturated fatty acids was significantly lower than that of normal cells |
| Key words: TLC GC phospholipid phosphatidylcholine fatty acid |