肝病患者血中T淋巴细胞亚群及NK细胞数量的变化

The Changes of T-lymphocyte subsets and Nature killer Cell Quantity in Patients with hepatic diseases

关键词:  T淋巴细胞亚群  自然杀伤细胞  慢性乙型肝炎  肝硬化  原发性肝癌  流式细胞仪

Key words:  T-lymphocyte subsets  nature killer  chronic virus hepatitis B infection  cirrhosis  primary heptocelluar carcinoma  flow cytometer

摘要:

为了研究慢性乙型肝炎、肝硬化及原发性肝癌(PHC)患者的免疫状态，观察T细胞亚群以及自然杀伤(NK)细胞数量在不同肝病中的变化情况，我们应用流式细胞仪测定了共147例肝病患者血中T细胞亚群和NK细胞数量。结果如下，与正常对照组相比较，各病例组CD3+及CD4+细胞数均减少，CD8+细胞数则升高(P＜0.05)，使CD4+/CD8+比值明显降低(P＜0.01)，其中，肝硬化与PHC组变化更为明显，PHC组的上述指标甚至与慢乙肝组之间也存在着差异(P＜0.05)。同时PHC组NK细胞数量明显低于正常对照组，甚至低于慢乙肝和肝硬化组(P＜0.05)。但其他三组间并无显著差别(P＞0.05)。因此用流式细胞仪检测肝病患者外周血中T淋巴细胞亚群及NK细胞数量具有快速、灵敏、准确等特点，其检验结果，对了解肝病患者的免疫状态、指导肝病的治疗及判断预后具有重要价值

Abstract:

To study the level of T-lymphocyte subsets and nature killer cell quantity in patients with chronic virus hepatitis Binfection.hepatocirrhosis and primary hepatocellular carcinoma(PHC),in order to analyze the immune status of these patients, we have determined periphery blood T-lymphocyte subsets and nature killercell quantity in 52 patients with chronic persistent hepatitis,49 patients with posthepatitis cirrhosis and 46 patients with primary hepatocelluar carcinoma, as well as 56 normal controls by flow cytometer. Compared with controls,the number of CD3+,CD4+ cells were decreased significantly in patients with hepatic diseases, while the number of CD8+ cells increased and the ratio of CD4+/CD8+ cell decreased(P<0.01), what is more,there was also some difference in the level of T-lymphocyte subsets between Chronic hepatits and primary hepatocellular carcinoma patients (P< 0.05 ). At the same time, the nature killer cell quantity in hepatocellular carcinoma,patients was significantly lower than that in hepatocellular carcinoma patients was significantly lower than that in normal controls, and even in chronic hepatits and cirrhosis groups(P< 0.05). So we can conclude that the methods to determine T-lymphocytesubsets and nature killer cell quantity by flow cytometer have such characteristics as fastness,accuracy and sensitivity, and the determination is valuable in studying the patients'immune status, curing liver disease and judging the prognosis.