生长环境对鳞状细胞癌分化及增殖的影响

The effection of environment on differentiation and proliferation of squamous cell carcinomas

关键词:  微环境  鳞状细胞癌  CK  PCNA

Key words:  microenviroment  squamous cell carcinoma  cytokeratin  proliferating cell nuclear antigen

摘要:

探讨不同部位鳞癌的细胞角蛋白，增殖细胞核抗原基因产物表达的差异性，对研究肿瘤局部生长环境对肿瘤实质细胞的影响具有一定的理论意义。方法：采用免疫组织化学LSAB法检测69例不同部位鳞癌(皮肤鳞癌9例、鼻咽鳞癌10例、喉鳞癌12例、食管鳞癌14例、宫颈鳞癌12例、肺鳞癌12例)的细胞角蛋白(cytokeratin,CK)、增殖细胞核抗原(prolirerating cell nuclear antigen,PCNA)基因产物的表达情况。结果：皮肤、食管鳞癌多为高分化鳞癌，鼻咽、肺鳞癌多为低分化鳞癌；CK在不同部位鳞癌的表达由强到弱依次为皮肤、食管、喉、宫颈、肺；CK阳性细胞在皮肤、食管鳞癌组织中由癌巢周边到中央表达逐渐增强，CK阳性细胞在鼻咽、肺鳞癌组织中散在分布；高分化鳞癌较低分化鳞癌表达强。PCNA的表达在鼻咽、肺鳞癌

Abstract:

To investigate the different expression of CK and PCNA proteins of squamous cell carcinoma(SCC)in various tissues. Methods: The expression of CK and PCNA antigen proteins was examined by immunohistochemical staining in 69 cases SCC(9 skin,10 laryngeal, 12 nasopharyngeal, 14 esophageal, 12 cervical and 12 lung SCC). Result: well differentiated SCC was mainly found in epidermal esophageal SCC, whereas poorly differentiated SCC was mainly found in nasopharyngeal and lung SCC. The strength of CK expression was as follows: skin, esophageal, laryngeal, cervical, nasopharyngeal and lung SCC. The number of CK proteins positive cells increased gradually in epidermal and esophageal SCC from the edge to the center of the cell nest,whereas diffused in nasopharyngeal and lung SCC. Significantly higher expression of PCNA proteins was found in nasopharyngeal and lung SCC compared with skin and esophageal SCC(P＜0.05). PCNA positive cells were observed mainly on the edge of the cell nest in epidermal and esophageal SCC, whereas diffused in nasopharyngeal and lung SCC. Conclusion: These results suggest that enviroment may play an important role in differentiation and proliferation of squamous cell carcinoma