E.coli DNA 对荷瘤小鼠血清中TNF-α、NO含量的影响

The effect of E. coil DNA on serum TNF-α、 NO in mice with tumor

关键词:  E.coli DAN  TNF-α  NO

Key words:  E.coli DNA  TNF-α  NO

摘要:

24只BALB/c小鼠中，8只作为正常对照组，其余16只荷瘤后，随机分为两组，E.coli DNA治疗组和TES对照组。荷瘤后第4 d予DNA治疗组、TES对照组小鼠皮下注射E.coli DNA(100μg/ml)或TES(0.1mol/L) 0.3 ml/只，隔日1次，共5次，荷瘤后第21d，摘眼球取血，制备血清，测定TNF-α、NO含量。结果显示：E.coli DNA可调节荷瘤机体的TNF-α、NO水平，防止TNF-α的异常增高及NO的显著下降，维持其在正常水平范围内，充分发挥其抗肿瘤免疫活性。这为细菌DNA作为一种新的生物反应调节剂提供了一些理论支持

Abstract:

Eight BALB/c mice were used as normal group,the other 16 mice were inoculated with HCaF16A3(106per mouse),then they were randomly divided into 2 groups,which were E.coli DNA group and TES group.From the 4th day after tumor inoculation,mice of DNA and TES group were injected subcutaneously with E.coli DNA(100μg/ml)or TES(0.1 mol/L)，0.3 ml per mouse,every other day, 5 times totally,At the 21st day,the eye was excised to take blood. Serum was made to measure TNF-α and NO.The results showed that E.coli DNA could modulate the serum TNF-α and NO,preventing TNF-α from being abnormally high and NO from declining greatly,E.coli DNA could also maintain TNF-α and NO in the nomal level to exert its antitumor immunal activity. These provided theoretical support to bacterial DNA as biological response modifier