一氧化氮在急性出血坏死性胰腺炎肠道损伤中保护作用的研究

Actions of nitric oxide in intestinal tract damaging following acute hemorrhagic necrotizing pancreatitis

关键词:  一氧化氮  急性出血坏死性胰腺炎  肠损伤

Key words:  nitric oxide(NO)  acute hemorrhshic necrotizing pancreatitis(AHNP)  intestinal damage

摘要:

探讨一氧化氮(NO)在急性出血坏死性胰腺炎(AHNP)时肠损伤的作用。方法：用去氧胆酸钠逆行注射法复制AHNP模型。将SD大鼠随机分为三组：假手术组(SOG)；急性出血坏死性胰腺炎组(APG)；L-NAME治疗组(LTG)。分别测定术后1、4、8、12 h各组血清淀粉酶和肠组织NO含量，对胰腺和小肠组织作病理检查，并进行病理组织评分。结果：SOG术后血清淀粉酶无明显变化，APG和LTG血清淀粉酶随时间推移呈上升趋势，各时相均高于SOG。NO含量APG各时相均高于LTG，胰腺和肠组织LTG在1、4h与APG无明显区别，8h时比APG严重。结论：①NO在AHNP时肠损伤中起保护作用；②NO在AHNP时对胰腺本身可能具有保护作用

Abstract:

To study the relationship between nitric oxide(NO)and intestin damages when acute hemorrhagic necrotizing pancreatitis(AHNP)occurs,and might provide some help to research and treat intestine damage of AHNP.Methods:Copying AHNP model with retrograde injection of deoxysodium cholate into pancreatic duct,SD rats were randomly divided into three groups:sham operation group(SOG);acute hemorrhagic necrotizing pancreatitis group(APG); L-NAME treatment group(LTG).Then serums amylaes and NO content of intestine tissue at the lst, 4th, 8th and 12th hour were measured. The pancreas and intestine tissues were examined pathologically. Results:There was no remarkable changes of serums amylase level in SOG. The serums amylase levels were higher in APG and LTG at every phase. NO content of intestine tissue was higher of APG than LTG at every phase. The pathological and histological changes of pancreas and intestine tissues of LTG was only a little different from APG at lst and 4th, but worse than APG at the 8th.Conclusions:NO has the protective function in intestinal damage process at the occurrence of AHNP and also has the occurrence function in pancreas at occurrence of AHNP