| 摘要: |
| 研究卵巢癌中是否存在nm23-H1基因突变及其与卵巢癌分期、组织学类型、分化程度的关系；方法：利用PCR-SSCP技术研究了正常卵巢、良性及恶性卵巢瘤中nm23-H1基因的5个外显子的突变情况；结果：正常卵巢、良性及交界性卵巢肿瘤未见突变，32例恶性卵巢瘤中9例有突变；低分化程度卵巢癌的突变率高于高、中分化程度的卵巢癌(P＜0.005)；浆液性卵巢癌的突变率高于其他组织学类型的卵巢癌(P＜0.005)； Ⅲ、Ⅳ期突变率与Ⅰ、Ⅱ期之间相比有显著性差别(P＜0.05)；有淋巴结转移的卵巢癌基因突变率高于无淋巴结转移卵巢癌(P＜0.05)；结论：nm23-H1基因突变易发生于分化程度较低、有淋巴结转移的晚期浆液性卵巢癌。 |
| 关键词: 卵巢癌 nm23-Hl基因 PCR-SSCP 突变 转移 |
| DOI：10.11724/jdmu.2000.03.01 |
| 分类号:R816.91 |
| 基金项目:辽宁省大连市卫生局资助项目 |
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| The mutations of nm23-H1 associated with malignancy of ovarian tumors |
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WANG Zhong-min1, CHANG De-an2
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1.Gynecology and Obstetrics Hospital of Dalian,Dalian 116033,China;2.Department of Gynecology and Obstetrics,the Second Affiliated Hospital,Dalian Medical University, Dalian 116027 China
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| Abstract: |
| Objective:To find out the relationship of mutations of nm23-H1 to stages, histological types and differentiation of ovarian tumors.Method:The polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP)analysis was performed to examine mutations in 5 exons of nm23-H1 gene in samples from normal ovaries,benign and malignant ovarian tumors. Results: Mutation was not found in normal ovaries,benign and borderline ovarian tumors, while was found in 9 out of 32 cases of malignant ovarian tumors(P＜0.05). The mutation rate of serous carcinoma was higher than mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma(P＜0.005). The mutation rate of carcinomas with stage Ⅲ or Ⅳ were higher than those with stage I or Ⅱ(P＜0.05).The mutation rate was higher in cases with lymph node metastases than those without lymph node metastases(P＜0.05). Conclusion:These results suggested that mutation of nm23-H1 gene tend to exist in advanced serous ovarian carcinomas with lymph node metastases |
| Key words: ovarian carcinoma nm |