定量测定甲硝唑注射液中内毒素及相关问题的探讨

Ouantitative deteremination of endotoxin in metronidazole injection and discussion about the related topics

关键词:  细菌内毒素检查法  亲和膜分离法

Key words:  Bacterial endotoxin test method  Affinity membrane isolation method

摘要:

采用细菌内毒素检查法和亲和膜分离法,定量检测了甲硝唑注射液中细菌内毒素,0.2%甲硝唑注射液稀释5倍测定,0.5%甲硝唑注射液稀释13倍测定(鲎试剂灵敏度0.125 EU//ml).用亲和膜分离法可直接测定,同细菌内毒素检查法比较接近,临床应用简捷,快速,方法准确可靠.

Abstract:

The bacterial endotoxin in metronidazole injection was quantitatively detected using bacterial endotoxin test method and affinity membrane isolation method,To be detected 0.2% Metronidazole injection was diluted by fivefold, 0.5% metronidazole injection by thirteenfold (The sensitivity of tachypleus amebocyte lysate 0.125 EU/ml).The endotoxin in the above injections can directly be measured by affinity membrane isolation method with the results close to that obtained by bacterial endotoxin method.Affinity membrane isolation method is characterized by being simple, rapid, accurate, reliable in clinical uses.