| 摘要: |
| 采用小鼠肝癌腹水瘤细胞系Hca-F25/CL-16A3,利用流式细胞术(Flowcytometry,FCM)研究了榄香烯对肿瘤细胞的作用、对Hca-F25/CL-16A3细胞周期的影响、DNA含量的分析以诱导细胞凋亡.结果表明:榄香烯可以明显抑制Hca-F25/CL-16A3细胞的增殖,有效地杀伤肿瘤细胞;榄香烯对GO/G1期细胞无明显作用,主要作用于S期,阻止由S期进入G2/M期,从而抑制肿瘤生长;可在GO/G1期前出现一个凋亡特异的AP峰. |
| 关键词: 流式细胞术 榄香烯 凋亡 |
| DOI：10.11724/jdmu.2000.01.19 |
| 分类号:R446.11+2 |
| 基金项目: |
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| The study of the antitumor activity of elemene by flow cytomnetry (FCM) |
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Zuo Yunfei,Zhang Yaozheng,Zheng Yun
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Department of Laboratory,Dalian Medical University,Dalian,China
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| Abstract: |
| In the review,author analyzed the antitumor activity of elemene preparation, influence for Hca-F25/CL-16A3，DNA content and the function with induction apoptosis with Hca-F25/CL-16A3 by flow cytometry(FCM). Result:Elemene could inhibit the growth of tumor abviously, kill the tumor cell. During period of the reaction，GO/G1phase did not change, elemene could arrest cell cycle from S phase to G2/M phase transtion and make it more in S phase. At last,inhibit the tumor cell,and become a AP-phase in front of GO/G1phase |
| Key words: flow cytometry elemene apoptosis |