| 摘要: |
| 目的:研究胃癌组织及相应癌旁组织中的端粒酶活性,探讨其作为胃癌肿瘤标记物的可能性.方法:采用PCR-ELISA方法检测36例胃癌组织及36例相应癌旁组织中端粒酶活性的表达.结果:36例胃癌组织中,30例端粒酶呈阳性,阳性率为83.3%;而36例癌旁组织中只有1例阳性.结论:端粒酶是特异性较强的恶性肿瘤基因标志,有可能成为胃癌早期诊断的理想标志物. |
| 关键词: 胃癌 端粒酶 PCR-ELISA 肿瘤标记 |
| DOI：10.11724/jdmu.2000.01.16 |
| 分类号:R446.61 |
| 基金项目: |
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| Telomerase activity in human gastric cancer |
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Yuan Hong
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Department of clinical laboratory,the First Affiliated Hospital,Dalian Medical University, Dalian,China
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| Abstract: |
| To investigate the telomerase activity in human gastric carcinorma and tumor-adjacent tissue,and the possibility of telomerase as biological marker of the carcinoma. Methods:The telomerase activity were examined in 72 human gastric pecimens, including 36 carcinoma and 36 tumor-adjacent tissues by PCR-ELISA.Results:Of 36 gastric carcinoma specimens, 30(83.3%)were positive but only 1 case(2.8%)was positive in 36 tumor-adjacent tissues for telomerase activity. Conclusion:Result suggests that telomerase is useful gene marker of gastric carcinoma and play an important role in early diagnosis of gastric carcinoma. |
| Key words: gastric carcinoma telomerase PCR-ELISA tumor marker |