| 摘要: |
| 肿瘤是一种由多种致癌基因功能的获得，抑癌基因功能的丢失以及环境因素共同决定的复杂疾病。肿瘤细胞的表型异质性、细胞生物学背景以及肿瘤微环境是决定肿瘤发展过程的关键因素。体外三维细胞培养（three-dimensional cell culture，3DCC）可以更好地模拟真实的肿瘤细胞微环境，对探索肿瘤的发生、侵袭以及转移的分子机制起到了决定性作用。本文将结合近年来相关文献，对3DCC的建立方法及其在恶性肿瘤中的应用进展作一综述。 |
| 关键词: 三维细胞培养 肿瘤 支架 肿瘤微环境 |
| DOI：10.11724/jdmu.2020.02.15 |
| 分类号:R73-3 |
| 基金项目: |
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| Application and advances of in vitro three-dimensional culture technique in cancer research |
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SUN Yuefeng1, JIANG Jian2, WANG Ling3, WANG Hong1
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1.Department of Spine Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;2.Department of Minimally Invasive Spine Surgery, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510000, China;3.Department of Oncology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
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| Abstract: |
| Tumor is a complex disease which is involved in a variety of oncogene activation, tumor suppressor gene deactivation and environmental factors. Phenotypic heterogeneity, biological context and microenvironment of tumor cells are the key factors to determine the development of tumor. Three-dimensional cell culture (3DCC) mimics the microenvironment of native tumor tissue and plays an important role in exploring the molecular mechanism of tumor initiation, invasion and metastasis. This review summarized the establishment methods of 3DCC and its application advances in malignant tumor research. |
| Key words: 3D cell culture tumor scaffolds tumor microenvironment |
