金樱子胶囊对四氯化碳诱导小鼠急性肝损伤的保护作用研究

Protective effect of Rosa laevigata Michx capsule against liver injury in mice induced by carbon tetrachloride

关键词:  金樱子胶囊  四氯化碳  肝损伤  氧化应激

Key words:  Rosa laevigata Michx capsule  CCl4  liver injury  oxidative stress

摘要:

目的　研究金樱子胶囊对四氯化碳（CCl₄）诱导小鼠急性肝损伤的保护作用。方法　昆明小鼠随机分为空白对照组、模型组和金樱子胶囊不同剂量（100 mg/kg、200 mg/kg、400 mg/kg，ig）给药组；给药7 d后，以腹腔注射0.4% CCl₄花生油溶液（0.1 mL/10 g）的方法建立急性肝损伤模型，HE染色观察小鼠肝脏组织病理学损伤，试剂盒检测小鼠血清谷草转氨酶（AST）、谷丙转氨酶（ALT）及肝组织超氧化物歧化酶（T-SOD）、丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-PX）含量。结果　金樱子胶囊能显著改善肝脏组织损伤，逆转CCl₄诱导的小鼠肝中SOD、GSH-PX、GSH活力下降，降低MDA含量，并显著降低血清中AST和ALT的含量。结论　金樱子胶囊可显著改善小鼠肝中氧化应激水平，对CCl₄诱导的小鼠急性肝损伤具有一定的保护作用。

Abstract:

ObjectiveTo observe the protective effect of Rosa laevigata Michx capsule against liver injury in mice induced by carbon tetrachloride (CCl₄).MethodsMice were randomly divided into the control group, the model group and different doses of Rosa laevigata Michx health food group［100 mg/(kg·d), 200 mg/(kg·d), 400 mg/(kg·d)］. After administration 7 d, we established the model of liver injury via intraperitoneal injection of 0.4% (CCl₄) (0.1 mL/10 g), andthen observe the pathology changes. Furthermore, we detected AST and ALT levels in serum, and MDA, SOD, GSH and GSH-PX levels in hepatic tissue.ResultsRosa laevigata Michx capsule could notably improved the injury of liver tissue and significantly increased liver SOD, GSH-PX and GSH activities and remarkably decreased MDA, AST and ALT in mice after treated by CCl₄.ConclusionsRosa laevigata Michx capsule has a protective effect against liver injury in mice induced by CCl₄ through regulating oxidative stress levels.