| 摘要: |
| 目的比较正常小鼠和荷乳腺癌小鼠骨髓中髓系抑制性细胞（MDSCs）的含量，探讨其产生免疫抑制功能的可能机制。方法建立荷乳腺癌小鼠模型，利用BD FACSAria Ⅲ流式分选仪分选正常小鼠组和荷乳腺癌小鼠组骨髓中的MDSCs（CD11b+ Gr-1+），比较其含量；q-PCR法检测两组MDSCs中MCT1及LDHB的mRNA水平。结果荷乳腺癌小鼠骨髓中MDSCs的比例为（48.92±3.13）%高于正常小鼠(25.13±1.95)%，差异有统计学意义（P<0.001）；荷乳腺癌小鼠MDSCs中MCT1的mRNA相对表达量(0.010±0.005)为正常小鼠（0.003±0.001）的3.33倍，差异有统计学意义（P<0.05）；荷乳腺癌小鼠MDSCs中LDHB的mRNA相对表达量(0.008±0.002)为正常小鼠（0.004±0.001）的2.00倍，差异极显著（P<0.01）。结论MDSCs在荷乳腺癌小鼠骨髓中的比例显著高于正常小鼠，其能量代谢关键分子MCT1、LDHB的表达也显著高于正常小鼠。MDSCs的能量代谢特点可能正是其发挥免疫抑制功能的重要基础。 |
| 关键词: MDSC MCT1 LDHB 能量代谢 免疫抑制 |
| DOI：10.11724/jdmu.2017.06.03 |
| 分类号:R73 |
| 基金项目:基金项目：福建省卫计委青年课题（2016-1-60）；2016年福建医科大学启航基金（2016QH042） |
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| Expression and significance of MCT1 and LDHB in MDSCs from bone marrow of breast cancer bearing mice |
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LAN Ruilong1,2, ZHANG Na3, CHEN Ruiqing1,2
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1.Central Laboratory of the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China;2.Fujian Key Laboratory of Individualized Active Immunotherapy,Fuzhou 350005,China;3.Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital,Fuzhou 350001,China
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| Abstract: |
| Objective To test the MDSCs ratios in bone marrow from normal and breast cancer bearing mice, and compare the mRNA levels of the key energy metabolism molecules-MCT1/LDHB and explore the possible mechanism of immunosuppression in MDSCs. Methods MDSCs (CD11b+ Gr-1+) in bone marrow was sorted by BD FACSAriaⅢ. MCT1/LDHB mRNA levels were detected with q-PCR. Results The MDSCs ratio (19.77±4.77)% in breast cancer bearing mice was much higher than that (0.84±0.18)% in normal group （P<0.001).Both MCT1 and LDHB mRNA of MDSCs in tumor bearing group were significantly higher than that in normal group （P<0.05 and P<0.01, respectively). Conclusions The energy metabolism could play an role in the immunosuppression of MDSCs. |
| Key words: MDSC MCT1 LDHB energy metabolism immunosuppression |
