| 摘要: |
| 目的 对罗氏e601电化学发光仪剩余试剂再利用进行评估。 方法 将HBsAb、HBcAb、HBeAg、HBeAb的混合剩余试剂，进行精密度、正确度验证并与新试剂进行比对及相关性分析。 结果 剩余混合试剂HBsAb、HBcAb、HBeAg、HBeAb水平1批内精密度分别为3.43、3.88、2.04、1.22，水平2批内精密度分别为1.22、2.40、1.09、1.66；HBsAb、HBcAb、HBeAg、HBeAb水平1批间精密度分别为5.17、4.55、3.70、3.74；水平2批间精密度分别为4.99、3.93、4.16、6.91；正确度本室测定结果与室间质评回报结果均相符、HBsAb相关系数r为 0.999；HBcAb、HBeAg、HBeAb两试剂盒的阳性符合率和阴性符合率均为100%。 结论 罗氏e601电化学发光仪剩余试剂可以混合使用，稳定性良好，能保证检测质量。 |
| 关键词: 罗氏e601 剩余试剂再利用 电化学发光 |
| DOI：10.11724/jdmu.2016.05.16 |
| 分类号:R446.69 |
| 基金项目: |
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| Recycling value of remaining reagent by Roche e601 electrochemiluminescence analyzer |
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YU Miao-chen1, PAN Li-yang2, ZHU Hong1
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1.Clinical Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;2.Department of Infectious Diseases and Endemic, Dalian City Center for Disease Control and Prevention, Dalian 116011, China
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| Abstract: |
| Objective To evaluate the recycling value of remaining reagent by Roche e601 electrochemiluminescence analyzer. Methods The precision, accuracy, comparison and correlation analysis were performed between original reagents and recycling reagents for HBsAb、HBcAb、HBeAg、and HBeAb. Results The intra batch precision results of level 1 were 3.43,3.88,2.04,1.22 respectively，the intra batch precision results of level 2 were 1.22,2.40,1.09,1.66 respectively；the inter batch precision results of level 1 were 5.17,4.55,3.70,3.74 respectively；the inter batch precision results of level 2 were 4.99,3.93,4.16,6.91 respectively.The accuracy result was consistent with the EQA result；the correlation coefficient of HBsAb was 0.999；the positive rate and negative coincidence rate（%）of HBcAb,HBeAg,HBeAb were 100%. Conclusion The remaining reagents can be combined and recycled by Roche e601, demonstrate good stability and ensure the quality of measurement. |
| Key words: Roche e601 recycling of remaining reagent electrochemiluminescence |
