冬凌草甲素对甲状腺癌BCPAP细胞增殖的抑制作用

Study of Oridonin inhibiting proliferation of thyroid carcinoma cell line BCPAP

关键词:  冬凌草甲素  甲状腺癌细胞株BCPAP  细胞增殖  细胞凋亡

Key words:  Oridonin  thyroid carcinoma cell line BCPAP  cell proliferation  cell apoptosis

摘要:

目的　研究冬凌草甲素对甲状腺癌BCPAP细胞增殖的抑制作用。方法　使用0、0.5、1、2、5、10、20、50、100 μmol/L浓度的冬凌草甲素处理甲状腺癌细胞株BCPAP 48 h后，用CCK8测定细胞增殖率，绘制增殖曲线，计算细胞半数抵制率（IC₅₀）。实验组加入接近IC₅₀浓度（10 μmol/L）的冬凌草甲素，对照组细胞正常培养36 h后，采用流式细胞术检测细胞凋亡，利用Western blot检测凋亡基因PARP、Erk1/2和p-Erk1/2蛋白的表达。结果　冬凌草甲素能显著抑制BCPAP细胞增殖，在2~20 μmol/L时，具有明显的量效关系。流式细胞术检测发现，与对照组相比，实验组细胞早期凋亡率（2.83±0.70）%，晚期凋亡率（1.43±0.31）%，总凋亡率（4.27±1.01）%，均明显升高（P<0.05）。Western blot发现，剪切后的PARP及p-Erk蛋白的表达水平分别上调了1.5倍和1.8倍，上调显著（P值均<0.05）。结论　冬凌草甲素可抑制甲状腺癌细胞株BCPAP增殖，其可能与诱导细胞凋亡有关。

Abstract:

Objective　 To investigate the mechanism of Oridonin on proliferation of BCPAP thyroid carcinoma. Methods　 Thyroid carcinoma cell line BCPAP was treated 48 h with Oridonin. CCK8 method was used to detect cell proliferation rate, flow cytometry used in apoptosis analysis and western blot method used to detect PARP and Erk1/2 expression. SAS 9.3 software was used in statistical analysis. Results　 Oridonin inhibited proliferation of BCPAP thyroid carcinoma significantly and had dose-dependent in middle-level concentration (2-20 μmol/L).The cells treated with oridonin showed higher apoptosis frequency than those non-treated (P<0.05). Spliced PARP and p-Erk1/2 expression increased in cells treated with oridonin. Conclusion　 Oridonin would inhibit proliferation of BCPAP by induction cell apoptosis.