半脾模型在裸鼠结直肠癌肝转移中的实验研究

Nude mice hemispleen method in hepatic metastases of colon cancer model

关键词:  裸鼠  结直肠癌  半脾模型  药物筛选模型

Key words:  [Key words]　nude mice  colon cancer  hemispleen model  drug screening model

摘要:

[摘要]　目的　建立稳定的裸鼠结直肠癌肝转移半脾模型。　方法　利用裸鼠脾脏的特殊解剖，建成半脾模型后，将人HT29结直肠癌细胞接种于近端半脾被膜下，远端半脾埋入皮下备治疗所需。采用不同浓度的肿瘤细胞（5×105/100 μL、1×106/100 μL及5×106/100 μL）或不同浓度的环磷酰胺（L-CTX和H-CTX），共分6组观察制模30天后的肝转移评分、淋巴结转移及血性腹水情况。　结果　人HT29结直肠癌细胞建立的肝转移半脾模型成瘤率和减瘤率皆为100%。L-CTX治疗组和H-CTX治疗组观察结果，发现随结直肠癌细胞浓度梯度的增高肝转移评分也增高，其不同浓度梯度间的差异有显著性意义（两组均为P<0.05）。L-CTX治疗组淋巴结转移和血性腹水明显少于H-CTX治疗组，其差异也有显著性意义（P<0.05）。人HT29结直肠癌细胞浓度梯度为1×106/100 μL制模组，显示其模型稳定性和抗肿瘤药物的敏感性最好。　结论　裸鼠结直肠癌肝转移半脾模型为结直肠癌肝转移的生物学机制和抗转移治疗提供了较为理想的实验模型。肝转移半脾模型为抗肿瘤药物体外筛选模型方法，其模型的建立能应用于抗肿瘤药物的筛选。

Abstract:

[Abstract] Objective To establish a feasible and stable hepatic metastases of colon cancer model with nude mice hemispleen method. Methods The spleen was divided, creating two hemispleens with their own vascular pedicle. HT29 human colon cancer cells were injected beneath the inferior hemispleen capsules of nude mice, establishing models of liver metastases in nude mice. Then removing the inferior hemispleen, the superior hemispleen was placed in the left s.c. pocket for treatmentinjection. The liver metastasis score, lymph node metastasis, and bloody ascites were studied after different concentrations of HT29 human colon cancer cells infection (5×105/100 μL, 1×106/100 μL and 5×106/100 μL) and different concentrations of cyclophosphamide (CTX) therapy (L-CTX and H-CTX).Results The rates of tumor formation and tumor reduce were all 100% in the hepatic metastases of colon cancer hemispleen models. The hepatic metastasis scores in the low concentration CTX group (L-CTX group) and high concentration CTX group (H-CTX group) both increased when increased theconcentrations of HT29 human colon cancer cells injection (P<0.05). The mice with lymph node metastases or bloody ascites were significantly fewer in L-CTX group than in H-CTX group (P<0.05). The hemispleen model with infection 1×106/100 μL HT29 human colon cancer cells showed better in model stability. Conclusion The hemispleen model are technically feasible and reproducible in researche on the metastatic mechanism and therapeutic development of colorectal cancer. The feasible and stable hemispleen model could be used in screening antitumor drugs.