| 摘要: |
| [摘要] 目的 探讨MICA基因第5外显子微卫星多态性与卵巢癌发病的关系。 方法 实验组包括95名临床已确诊卵巢癌患者，对照组包括99名无血缘关系的辽宁汉族健康女性。采用PCR-毛细管电泳方法检测被试MICA基因第5外显子微卫星的基因型。 结果 两组均检测出5种等位基因，分别为A4（179 bp）、A5(182 bp)、A5.1（183 bp）、A6（185 bp）和A9（194 bp）。正常对照组MICA第5外显子A5.1等位基因的频率最高，为33.8%。实验组和对照组MICA第5外显子A5等位基因的频率分别为31.1%和19.2%，差异有显著性意义（P<0.01）。 结论 MICA基因第5外显子A5等位基因增加了卵巢癌的发病风险。 |
| 关键词: MICA基因 人类白细胞抗原 微卫星多态性 卵巢癌 |
| DOI：10.11724/jdmu.2015.05.05 |
| 分类号: |
| 基金项目:基金项目：大连市科技计划项目（2010J21DW035） |
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| Association of microsatellite polymorphism of MICA gene with susceptibility to ovarian cancer |
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GUO Lian-ying 1, QI-Zheng 2 ,WANG Hua-li 3, LIU Gang 41,2,3,4
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1.Department of Pathophysiology，Dalian Medical University，Dalian 116044，China;2.The High School Affiliated to Liaoning Normal University, Dalian 116023, China;3.Department of Gynecology, Dalian Obstetric and Gynecological Hospital，Dalian 116033，China;4.The Second Affiliated Hospital of Dalian Medical University, Dalian 116027,China
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| Abstract: |
| [Abstract] Objective To explore the relationship between microsatellite polymorphism of MICA gene exon 5 and the risk of ovarian cancer. Methods A total of 95 ovarian cancer patients and 99 unrelated healthy people were recruited in this study. PCR-capillary electrophoresis method was used to detect the microsatellite genotypes of MICA gene exon 5. Results Five alleles were observed in both groups, A4（179 bp）,A5(182 bp),A5.1（183 bp）, A6（185 bp）and A9（194 bp）, respectively. In the control group, A5.1 was the most common allele (33.8%). The frequency of A5 allele of MICA gene in ovarian cancer patients was significantly higher than that in controls (31.1% vs. 19.2%, P<0.01). Conclusion A5 allele of MICA gene exon 5 could be associated with high risk of ovarian cancer. |
| Key words: [Key words] MICA gene HLA microsatellite polymorphism ovarian cancer |
