| 摘要: |
| [摘要] 目的 了解鞍山地区丙型肝炎病毒（HCV）基因分型的情况。 方法 采用型特异性引物逆转录巢式PCR法对鞍山地区抗HCV和HCV RNA均阳性的患者共189例进行基因分型。 结果 在189份抗HCV和HCV RNA均阳性血清中基因型分别检测出了1b和2a型，未发现混合型感染，其中11份为阴性。 结论 鞍山地区基因型有1b型和2a型两种型，未发现其他亚型，1b型（53％）和2a型（47％）基本相等。 |
| 关键词: 丙型肝炎病毒 基因型 亚型 型特异性引物 逆转录巢式PCR法 |
| DOI：10.11724/jdmu.2015.04.11 |
| 分类号: |
| 基金项目:基金项目：辽宁省科技厅项目（2010225034） |
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| Distribution of Hepatitis C virus genotype in Anshan Central Hospital |
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ZHANG Yan-hong 1, XIE Ying 1, XIE Chen 1, LIU Wen-zhi 1, WEI Yue 21,2
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1.Department of Infectious Disease, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027,China;2.Department of Infectious Disease, Anshan Central Hospital, Anshan 114001,China
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| Abstract: |
| [Abstract] Objective To investigate the distribution of HCV genotype in Anshan. Methods HCV genotype was determined by RT-nested-PCR in 189 cases of anti-HCV and HCV RNA positive patients in Anshan. Results In the serum of the 189 anti-HCV and HCV RNA positive patients, 1b and 2a were detected. No mixed genotype was found. 11 cases were negative. Conclusion There are only two HCV genotypes, 1b and 2a, found in Anshan. They have almost equal distribution with 53% 1b and 47% 2a, respectively. |
| Key words: [Key words] Hepatitis C virus genotype subtype primer deduced RT-nested-PCR |
