Survivin和MUC2在萎缩性胃炎和胃癌组织中的表达及意义

Expression and significance of Survivin and MUC2 in human atrophic gastritis and gastric carcinoma

关键词:  Survivin  MUC2  胃肿瘤  肠化生  组织芯片技术  免疫组化

Key words:  [Key words]　Survivin  MUC2  stomach neoplasm  intestinal metaplasia  tissue microarray  immunohistochemistry

摘要:

[摘要]　目的　探讨生存素（Survivin）和黏蛋白2（mucin 2，MUC2）的表达与萎缩性胃炎伴肠化生及胃癌的关系。　方法　构建含30例正常胃黏膜、30例萎缩性胃炎伴肠化生和41例胃癌的组织芯片，用免疫组化方法（S-P法）检测组织芯片中Survivin和MUC2的表达。　结果　Survivin和MUC2表达阳性率在正常胃黏膜组织中为7.14%和0%；在萎缩性胃炎伴肠化生胃黏膜中为21.43%和75.00%；在胃癌中为77.78%和13.89%。二者在不同胃黏膜阳性表达率总体差异有显著性意义（P<0.001）。胃癌组织中Survivin表达与Lauren分型、浸润深度、TNM分期有关（P<0.05）。MUC2表达与Lauren分型、分化程度有关（P<0.05）。二者表达均与患者性别、年龄、淋巴结转移、病变部位、肿瘤大小无关(P>0.05)。Survivin与MUC2表达间无明显相关性（r=0.215,P=0.209）。　结论　Survivin和MUC2表达在胃黏膜肠化生及胃癌发生中发挥一定作用。

Abstract:

[Abstract]　Objective 　To investigate expression of Survivin and mucin 2(MUC2),and their correlation with atrophic gastritis with intestinal metaplasia and gastric carcinoma．　Methods　30 normal gastric mucosa, 30 tissues with atrophic gastritis with intestinal metaplasia，41 tissues with gastric carcinoma were detected with immunohistochemistry. The expressions of survivin and MUC2 in tissue microarray were studied．　Results　Two normal gastric mucosa expressed Survivin(7.14%),but MUC2 was not expressed in the normal gastric mucosa. The positive expression rate of survivin was 21.43% and MUC2 was 75.00% in atrophic gastritis with intestinal metaplasia. The positive expression rate of Survivin was 77.78%, MUC2 was 13.89% in gastric carcinoma .The positive expression rate of Survivin and MUC2 in different gastric mucosa was of statistical significance（P<0.001）.The expression of Survivin in gastric carcinoma showed statistically significant difference（P<0.05）in different Lauren classification、depth of invasion and TNM categories. The expression of MUC2 in different Lauren classification,tumor differentiation was statistically significant difference（P<0.05）. The expression of Survivin and MUC2 in gastric carcinoma was not associated with gender,patients age, lymph node metastasis, tumor location, tumor size(P>0.05). The expression of Survivin shows no obvious correlation with MUC2（r=0.215,P=0.209）. 　Conclusion　The expressions of survivin and MUC2 might play an important role in the development of gastric carcinoma.