DESI2基因在肝细胞癌中的表达及其对HepG2细胞凋亡的影响

Expression of DESI2 gene in hepatocellular carcinoma and the effect on apoptosis of HepG2 cell

关键词:  DESI2基因  肝细胞癌  HepG2细胞  凋亡

Key words:  [Key words]　DESI2 gene  hepatocellular carcinoma  HepG2 cell  apoptosis

摘要:

[摘要]　目的　研究DESI2基因在肝细胞癌中的表达及其对肝癌细胞的影响。　方法　Real-time PCR检测53例肝细胞癌与相应癌旁组织中DESI2 mRNA表达。构建DESI2基因真核表达载体pcDNA3.3-DESI2，转染HepG2细胞。Western blot检测转染后HepG2细胞DESI2蛋白的表达，流式细胞术双标记法检测细胞凋亡率。　结果　与癌旁组织相比，DESI2 mRNA在肝细胞癌组织中表达下调63.95%。转染DESI2基因真核表达载体pcDNA3.3-DESI2后，HepG2细胞DESI2蛋白表达明显增加，细胞凋亡率明显增加。　结论　DESI2基因在肝细胞癌中表达下调，其表达上调能够促进肝癌细胞凋亡。

Abstract:

[Abstract] Objective　To Study on the expression and the effect of DESI2 in hepatocellular carcinoma.　Methods　Expression of DESI2 mRNA was detected in 53 cases of hepatocellular carcinoma and pericarcinoma tissues by real-time PCR. pcDNA3.3-DESI2 was constructed, then was transfected into HepG2 cells. Expression of DESI2 protein was detected by Western blot in HepG2 cells after transfecting pcDNA3.3-DESI2, the apoptosis of the transfected cells was detected by double labeling flow cytometry.　Results〖WTBZ〗 Compared with pericarcinoma tissues, the expression of DESI2 mRNA in hepatocellular carcinoma tissues decreased 63.95%. After transfection, the expression of DESI2 protein was significantly up-regulated, the apoptosis of the transfected cells increased significantly.　Conclusion　Expression of DESI2 mRNA decreased in hepatocellular carcinoma tissues. The over-expression of DESI2 gene can increase the apoptosis of HepG2 cells.