不同抗癫痫药物对幼年癫痫大鼠海马胶质原纤维酸性蛋白的影响

Effects of different anti-epileptic drugs on GFAP expression in immature aat Hippocampus with temporal epilepsy

关键词:  幼年大鼠  颞叶癫痫  左乙拉西坦  丙戊酸钠  蝎毒耐热蛋白  匹罗卡品

Key words:  [Key words] 　immature rats  temporal epilepsy  levetiracetam  sodium valproate  SVHRP  pilocarpine

摘要:

目的　探讨左乙拉西坦（levetiracetam, LEV）、丙戊酸钠（sodium valproate, VPA）和蝎毒耐热蛋白(scorpion venom heat resistant protein, SVHRP）对幼年癫痫大鼠海马胶质原纤维酸性蛋白（glia fibrillary acidic protein, GFAP）的影响。方法　生后2周SD大鼠共40只，分为（1）正常对照组：正常大鼠1次/d腹腔注射生理盐水1周；（2）癫痫模型组：应用匹罗卡品制作颞叶癫痫模型， 癫痫造模后，1次/d腹腔注射生理盐水1周；（3）LEV治疗组：癫痫造模后1次/d LEV灌胃（150 mg/kg）1周；（4）VPA治疗组：癫痫造模后1次/d VPA（250 mg/kg）灌胃1周；（5）SVHRP治疗组：癫痫造模后腹腔注射SVHRP（0.01 mg/kg），连续1周。用药后行免疫组化检测GFAP。结果　癫痫模型组海马齿状回GFAP免疫阳性的星形胶质细胞分别与LEV治疗组、VPA治疗组和SVHRP治疗组相比明显增生（P<0.05）。尼氏染色结果显示LEV治疗组、VPA治疗组和SVHRP治疗组与癫痫模型组比较，海马CA3区神经元损伤较小。结论　幼年大鼠癫痫发作可能与海马星形胶质细胞增生有关， LEV、VPA 、SVHRP的抗癫痫作用可能与抑制海马星形胶质细胞增生有关，但其具体作用及机制仍需进一步探讨。

Abstract:

[Abstract] Objective　To explore the effects of levetiracetam(LEV), sodium valproate (VPA) and scorpion venom heat resistant protein(SVHRP) on GFAP expression in immature rat hippocampus with temporal epilepsy (TE). Methods　SD rats (n=40) aged two weeks were randomly into five groups which were normal group, EP group, LEV group, VPA group and SVHRP group. Rats were injected with 10% pilocarpine to construct TE model. Assigned to receive intraperitoneal injection with either normal saline (EP group afer TE and normal group)，LEV (LEV group) or VPA(VPA group) with intragastric given LEV（150 mg/Kg）or VPA（250 mg/kg）for one week. Rats were given SVHRP（0.01 mg /kg）by intraperitoneal injection in SVHRP group daily for one week. GFAP in hippocampus was studied by immunohistochemistry. Result　Examination of Nissl-stained slides revealed that severe neuronal damage in the neurons of hippocampas CA3 subfield. However, SVHRP group, as well as LEV group and VPA group showed less damage in the CA3 subfield. The expression of GFAP in EP group was stronger than in SVHRP group,LEV group and VPA group respectively in the DG of hippocampus (P<0.05). Conclusions　Immature rats with TE is possibly related to the proliferation of astrocytes. The proliferation of rat hippocampal astrocytes could be inhibited by VPA，LEV and SVHRP，whose antiepileptic effect is probably mediated by inhibition of hippocampal astrocyte proliferation，but theirs mechanisms are still further studied.