大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用

Anti-peroxidative effects of SI-SPIO nanoparticles in liver fibrotic rats

关键词:  大豆异黄酮  肝纤维化  过氧化损伤

Key words:  [Key words]　soybean isoflavones  liver fibrosis  peroxidative damage

摘要:

目的　观察大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用。　方法　　采用四氯化碳灌胃法建立肝纤维化大鼠模型，随机分成对照组、肝纤维化组、空白纳米颗粒组（SPIO组）、大豆异黄酮组（SI组）、载药纳米颗粒组（SI-SPIO组），每组24只。对照组给予生理盐水尾静脉注射，其他各组除四氯化碳灌胃外，分别给予生理盐水尾静脉注射、SPIO、大豆异黄酮和SI-SPIO载药纳米颗粒尾静脉注射治疗，观察对照组和治疗组之间肝脏生化指标、过氧化损伤指标和肝脏组织学变化。　结果　　8周后对各组大鼠的肝脏生化指标和肝纤维化程度进行评估，SI组和SI-SPIO组明显优于肝纤维化组和SPIO组；过氧化指标检测，与对照组相比，肝纤维化组和SPIO组MDA明显升高（2.45±0.77，2.69±1.01，P<0.05），GSH-Px和SOD明显降低（300.74±16.25，299.18±15.57和409.77±20.46，431.56±19.25，P<0.05）；与肝纤维化组相比，SI组和SI-SPIO组MDA明显降低（0.85±0.12，0.90±0.02，P<0.05），GSH-Px和SOD明显升高（340.25±11.85，366.45±14.83和467.27±16.11，485.75±22.54，P<0.05）。SI-SPIO组过氧化指标较SI组改善更为明显（P<0.05）。　结论　　大豆异黄酮具有抗氧化作用，能够抑制大鼠肝纤维化形成，载药纳米有助于药效的发挥。

Abstract:

[Abstract] Objective　To investigate the anti-peroxidative effects of SPIO nanoparticles as a drug delivery system when combined with soybean isoflavones (SI-SPIO). 　Methods　The liver fibrosis models of rats were established by CCL4 gavage. 120 rats were random grouped as control, fibrosis, SPIO tail vein injection, isoflavone treatment and SI-SPIO treatment group. Normal saline injections were given in control group, CCL4 gavage were given in other groups, as well as NS injection, SPIO, SI and SI-SPIO injections to 24 rats of each group, respectively. Biochemical and histological indicators were analyzed. Peroxidative indicators were detected as well. 　Results〖WTBZ〗 Liver fibrosis was significantly improved in SI and SI-SPIO groups vs. control both in biochemical and histological aspects. Peroxcidative indicators such as MDA increased in liver fibrotic and SPIO groups （2.45±0.77, 2.69±1.01, P<0.05）, GSH-Px and SOD decreased（300.74±16.25, 299.18±15.57 and 409.77±20.46, 431.56±19.25, P<0.05）, SI administration reduced MDA （0.85±0.12，0.90±0.02, P<0.05）, increased GSH-Px and SOD in SI and SI-SPIO groups（340.25±11.85, 366.45±14.83 and 467.27±16.11, 485.75±22.54, P<0.05）. SI-SPIO group presents much more efficiency comparing with SI group （P<0.05）. 　Conclusion〖WTBZ〗 SI has anti-peroxidative effect and inhibits liver fibrosis in rats. SPIO nanoparticles make the effects more efficient as a drug delivery system.