局部应用神经生长因子对坐骨神经损伤模型大鼠修复与再生的影响

Department of Osteology, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China

关键词:  神经生长因子  周围神经再生  坐骨神经

Key words:  NGF peripheral  nerve regeneration  sciatic nerve

摘要:

目的　探讨神经生长因子局部给药对周围神经损伤后的修复与再生的影响。方法　SD大鼠48只，采用1%戊巴比妥钠30 mg/kg腹腔内麻醉。距梨状肌下缘远侧约1.5 cm处切断坐骨神经，切除远端1~2 mm，采用无创伤线（10/0）做神经外膜+束膜缝合。A组：局部注入2.5s神经生长因子0.3 μL。B组：坐骨神经缝合处注入生理盐水0.3 μL。术后2、4、6、8周进行动物行为学观察，光镜观察，8周时神经电生理检测、电镜观察，观察局部给药对周围神经损伤后修复与再生的影响。结果　A组术后患肢运动功能恢复情况优于B组，在取材时发现A组缝合处神经愈合良好，B组有1例神经瘤和1例感染。A组神经传导速度快[（11.04±0.34）m/s]、潜伏期短[(1.84±0.16)ms]，有髓神经纤维髓鞘较厚、直径较大、数量多、排列规则，再生良好。B组神经传导速度慢 [(8.94±0.37)m/s]、潜伏期长[(2.19±0.25)ms]，有髓神经纤维髓鞘较薄、直径较小、数量少、排列不规则，再生较差。A组优于B组（P<0.05）。结论　NGF局部给药具有明显的促进周围神经损伤后的修复与再生作用。

Abstract:

Object In order to understand the effect of NGF on the regeneration and to offer experimental evidences of clinical application of the peripheral nerve injury repairment. Methods Rat of sciatic nervous injury was used as experimental animal in this study.48 adult healthy rats(200-220g)were divided into two groups randomly,A group:NGF injection group in the peripheral nerve injury (24),B group: N.S injection group the peripheral nerve injury (24). The specimens were gained after 8 weeks. Results The results were as follow:1.Visual observation:A group,thedefected nerves were healed smoothly andquickly, B group was found onenerve tumour, one infection.2.Lm observation and axon computer analysis:in the A group, nerve fibers well developed and axons were inorder, no remarkable difference between axons count and nerve diameter,which were superior to B group (P<0.05).3.The nerve conduction velocity of A group were faster than that of B group, Latency were shorter in A group. Conclusion With continous using NGF in part of the peripheral nerve injury had enhancement effect to regeneration of peripheral nerve.