鼠胎膜修复大鼠腭裂的实验研究

Experimental study on cleft palate repair of rat using rat fetal membrane

关键词:  腭裂；动物模型；腭裂整复术；胎膜  大鼠

Key words:  cleft palate  animal model  cleft palate repair  fetal membrane  rat

摘要:

目的　以鼠胎膜对腭裂进行整复手术，研究修复腭裂的效果。方法　建立腭裂动物模型实验。30只SD大鼠按照腭部裂隙大小分为3组，每组10只。a组：长7 mm×宽0.9 mm；b组：长7 mm×宽1.1 mm；c组：长7 mm×宽1.3 mm。术后12周处死所有大鼠，观察裂隙变化。手术关闭裂隙实验。10只SD大鼠分为3组。A组大鼠5只，手术造腭裂并以新鲜双层胎膜关闭裂隙；B组大鼠3只，手术造腭裂，令裂隙自行生长；C组2只作为空白对照组。术后4周处死动物，大体观察；制作切片，行组织学观察。结果　腭裂动物模型成功建立，确立b组7 mm×1.1 mm为临界缺损。A组大鼠腭裂得到成功修复，口鼻腔黏膜分别愈合，完全覆盖腭裂，镜下见腭裂处口腔黏膜层次清楚，存在黏膜下层，顶部有完整的鼻腔黏膜。结论　鼠胎膜对SD大鼠腭裂具有良好的修复效果。

Abstract:

Objective 　To explore the feasibility of repairing the defect in cleft palate using fetal membrane in a rat model. 　Methods 　To establish the animal model of cleft palate. Thirty SD rats were randomly divided into three groups with 10 rats in each group according to the different size of man-made cleft, group a: 7 mm×0.9 mm, group b: 7 mm×1.1 mm, group c: 7 mm×1.3 mm.Animals were sacrificed at the end of 12 weeks and the changes of clefts were compared. Repairing experiment for cleft palate. Ten SD rats were randomly divided into three groups, group A was underwent reduction with 5 rats, group B was only performed model operation with 3 rats, group C was blank with 2 rats.All rats were sacrificed at the end of 4 weeks and the gross and histological observation was respectively carried out. 　Results 　The animal model of cleft palate was successfully established. Group b was determined to be the animal model group for critical-sized defect. Cleft palate was repaired successfully in group A with fetal membrane of rat, the structure of palate mucosa was similar to that of the group C in gross and histology. 　Conclusion 　The tissue defect of cleft palate in a rat model can be repaired with rat fetal membrane, which is valuable for repairing human cleft palate in future.