前列腺癌组织P504S、E-Cadherin和bcl-2蛋白表达及意义

Significances of P504S, E-Cadherin and bcl-2 protein expression in prostate carcinoma

关键词:  前列腺癌  免疫组织化学  P504S  E-Cadherin  bcl-2

Key words:  protaste carcinoma  immunohistochemistry  P504S  E-Cadherin  bcl-2

摘要:

目的　 分析前列腺癌中P504S、E-Cadherin和bcl-2蛋白的表达情况，探讨其在前列腺癌诊断及预后判断中的意义。 方法　 应用免疫组织化学（Max Vision）法检测45例前列腺腺泡腺癌、6例前列腺高级别上皮内肿瘤及10例前列腺增生组织中P504S、E-Cadherin和bcl-2蛋白的表达情况。结果　 P504S蛋白阳性率前列腺癌组（84.4%）明显高于前列腺高级别上皮内肿瘤组（50.0%）和前列腺增生组（0%）（P<0.05）；E-Cadherin蛋白阳性率前列腺癌组（42.2%）和前列腺高级别上皮内肿瘤组（33.3%）明显低于前列腺增生组（100%）（P<0.05）；bcl-2蛋白阳性率前列腺癌组（60.0%）明显高于前列腺高级别上皮内肿瘤组（16.7%）和前列腺增生组（20.0%）（P<0.05）。前列腺癌Gleason 8～10分组E-Cadherin蛋白的表达率（31.8%）明显低于2～4分组（83.3%）（P<0.05）； 8～10分组bcl-2蛋白的表达率（68.2%）明显高于2～4分组（33.3%）（P<0.05）。前列腺癌Gleason8～10分组（18.2%）和5～7分组（17.6%）E-Cadherin蛋白的强阳性表达率明显低于2～4分组（83.3%）（P<0.05）。前列腺癌组P504S蛋白阳性同时E-Cadherin蛋白阴性的比率（51.1%）明显高于前列腺增生组（0%）（P<0.05）；前列腺癌组P504S蛋白阳性同时bcl-2阳性的比率（57.8%）明显高于前列腺增生组（0%）（P<0.05）。结论　 P504S高表达、bcl-2高表达有助于前列腺癌的诊断；联合检测P504S和E–Cadherin，P504S和bcl-2的表达，可以提高诊断前列腺癌的准确性。

Abstract:

Objective 　To study the possibility of P504S, E-Cadherin and bcl-2 protein as a marker to diagnose prostate carcinoma. 　Methods 　The expressions of these proteins were detected by immunohistochemistry (Max Vision) method. 　Results 　The expression rate of P504S in prostate carcinoma group (84.4%) was significantly higher than high prostate intraepithelial neoplasia group (50.0%) and prostate gland proliferation group(0%) (P<0.05); That of E-Cadherin in prostate carcinoma group (42.2%) and high prostate intraepithelial neoplasia group (33.3%) were significantly lower than prostate gland proliferation group (100%) (P<0.05); That of bcl-2 in prostate carcinoma group (60.0%) was significantly higher than high prostate intraepithelial neoplasia group (16.7%) and prostate gland proliferation group (20.0%) (P<0.05). The expression of E-Cadherin in 8-10 score group (31.8%) was significantly lower than 2-4 score group (83.3%)(P<0.05); The expression of bcl-2 in 8-10 score group (68.2%) was significantly higher than 2-4 score group (33.3%) (P<0.05). The strong expression rate of E-Cadherin in 8-10 (18.2%) and 5-7 (17.6%) score groups were signifi cantly lower than 2-4 score group (83.3%) (P<0.05). Both P504S positive and E-Cadherin negative expression rate in prostate carcinoma group (51.1%) was significantly higher than prostate gland proliferation group (0%) (P<0.05). Both P504S and bcl-2 positive rate in prostate carcinoma group (57.8%) was significantly higher than prostate gland proliferation group (0%) (P<0.05). 　Conclusion 　The high expression of P504S, bcl-2 protein may be helpful to diagnose prostate carcinoma. The diagnosis accurate of prostate carcinoma could be increased by detect the expressions of P504S and E-Cadherin, P504S and bcl-2 proteins.