调合消毒法对石膏模型消毒效果的实验研究

Experimental study of disinfection effect of gypsum mixed with disinfectant

关键词:  调合法  石膏模型  消毒  细菌

Key words:  mixed method  gypsum model  disinfection  bacteria

摘要:

[目的] 探讨消毒液调拌超硬石膏对沾染微生物石膏模型的消毒效果。[方法] 将硅橡胶印模按所染菌种分为表皮葡萄球菌组,白色念珠菌组和绿脓杆菌组。并设立不染菌的阴性对照组。用2%戊二醛、0.5% 84消毒液、0.5%碘伏和无菌蒸馏水调拌超硬石膏，于印模内灌注石膏模型，1 h后脱模。无菌蒸馏水调拌组为阳性对照组。将各石膏模型试件浸入稀释液中洗下余菌、接种、培养并进行CFU计数,计算各组的杀菌率。[结果] 2%戊二醛、0.5?消毒液、0.5%碘伏调拌超硬石膏对表皮葡萄球菌、白色念珠菌、绿脓杆菌的杀菌率达100%。[结论] 用2%戊二醛、0.5?消毒液、0.5%碘伏调拌超硬石膏对沾染表皮葡萄球菌、白色念珠菌、绿脓杆菌的石膏模型消毒合格。

Abstract:

[Objective] To evaluate the effects of disinfection on contaminated gypsum models mixed with disinfectant in vitro. [Methods] Silicone rubber impressions were divided into the group of Staphylococcus epidermidis, Candida albicansand and Pseudomonas aeruginosa. The negative control group with no bacterial contamination was set up. Silicone rubber impressions were poured with gypsum power mixed with 2% glutaraldehyde, 0.5?, 0.5% iodophor and sterile distilled water. One hour later gypsum models were released from silicone rubber impressions.The group of sterile distilled water was positive control group. All specimens were immersed in diluent respectively. Colony forming units were countered after culture and were used to calculate the sterilization ratio. [Results] For Staphylococcus epidermidis、Candida albicans and Pseudomonas aeruginosa, 100% reduction of colony forming units was obtained with gypsum mixed with 2% glutaraldehyde，0.5% 84 and 0.5% iodophor. [Conclusion] Gypsum mixed with 2% glutaraldehyde，0.5% 84，0.5% iodophor can achieve an efficient effect on the gypsum models contaminated by Staphylococcus epidermidis, Candida albicans and Pseudomonas aeruginosa.