| 摘要: |
| [目的] 了解CTX-M-15基因与大肠埃希菌和肺炎克雷伯菌对头孢他啶耐药的关系。[方法]筛选ESBLs阳性且对头孢他啶耐药的菌株为实验组，对头孢他啶敏感而对头孢曲松耐药的菌株为对照组。多重PCR方法检测CTX-M-14,CTX-M-15基因。[结果]实验组CTX-M-15基因检出率高达75.9%，单纯CTX-M-15基因为40.6%，同时存在CTX-M15、CTX-M-14基因为35.2%。而对照组检出率分别为：17.7%、5.9%、11.8%。[结论]CTX-M-15基因是大肠埃希菌和肺炎克雷伯菌对头孢他啶耐药的主要机制，建议临床合理使用抗生素，避免引起流行。 |
| 关键词: CTX-M-15 头孢他啶耐药 大肠埃希菌 肺炎克雷伯菌 |
| DOI：10.11724/jdmu.2012.05.20 |
| 分类号: |
| 基金项目: |
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| CTX-M-15 gene detected in ceftazidime-resistant E. coli and Klebsiella pneumoniae isolates |
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LI Rui-hua 1, NIE Da-ping 1, HE Li-min 21,2
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1.Department of Clinical Laboratory,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;2.Laboratory medicine institute,Dalian Medical University,Dalian 116044,China
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| Abstract: |
| [Objective] To investigate the relationship of CTX-M-15 gene to ceftazidime resistance of Escherichia coli and Klebsiella pneumoniae.[Methods] CTX-M-15，CTX-M-14 genes in ceftazidime-resistant or susceptible, producing ESBL Escherichia coli and Klebsiella pneumoniae were detected by Multiplex-PCR. [Results] In ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates, the total positive rate of CTX-M-15 gene was 75.9%, the positive rate of alone CTX-M-15 gene was 40.6% and the rate of co-existence of CTX-M-15,14 genes was 35.2%. In ceftazidime - susceptible Escherichia coli and Klebsiella pneumoniae isolates they were 17.7%, 5.97% and 11.89% respectively. [Conclusion] CTX-M-15 gene is the major mechanism of ceftazidime resistance in Escherichia coli and Klebsiella pneumoniae. |
| Key words: CTX-M-15 ceftazidime-resistant Escherichia coli Klebsiella pneumoniae |
