| 摘要: |
| [目的]比较8种黄酮对血小板聚集的抑制作用并探讨其构效关系。[方法]采用体外实验方法，测定黄酮类化合物对ADP诱导大鼠血小板聚集的抑制作用，并计算其抑制率。[结果]槲皮素、芦丁、异槲皮素、橙皮素、柚皮素、橙皮苷、柚皮苷和淫羊藿苷对血小板聚集的抑制率分别为(68.33±2.43)%、(55.34±2.09)%、(52.27±4.65)%、(50.80±7.03)%、(33.07±2.09)%、(31.28± 4.65)%、(22.91±1.68)% 和(20.94±3.20)%，8种黄酮类化合物对血小板聚集均呈现抑制作用。 与对照组（不加黄酮类化合物）相比，差异均有显著性意义（P<0.05）。[结论]黄酮类化合物对血小板聚集有明显抑制作用，A环C7位和C环C3位的羟基是该类化合物抑制血小板聚集发挥作用的重要功能基团。 |
| 关键词: 黄酮类化合物 血小板聚集 结构 功能 |
| DOI：10.11724/jdmu.2012.05.02 |
| 分类号: |
| 基金项目:国家自然科学基金(81100722, 81171957);辽宁省教育厅科研项目(2008S077)；辽宁省自然科学基金(20082158) |
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| Structure-function study of flavonoid phytochemicals on platelet aggregation |
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GUO Yi-meng 1, GUO Chun-mei 2, LIU Shu-qing 1, YU Hong-shan 3, SUN Ming-zhong 21,2,3
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1. Department of Biochemistry, Dalian Medical University, Dalian 116044, China;2. Department of Biotechnology, Dalian Medical University, Dalian 116044, China;3. School of Bioengineering, Dalian Polytechnic University, Dalian 116034, China
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| Abstract: |
| [Objective] To compare the inhibitory effects of eight flavonoid phytochemicals on platelet aggregation and also explore the structure-function association. [Methods] The inhibitory effects of eight flavonoids on ADP- induced rat platelet aggregation were studied in vitro and obtained their inhibiting percentages. [Results] The results indicated that all the eight flavonoids including quercetin, rutin, isoquercitrin, hesperetin, naringenin, hesperidin, naringin and icariin could inhibit the ADP-induced rat platelet aggregation by (68.33±2.43)%, (55.34±2.09)%, (52.27±4.65)%, (50.80±7.03)%, (33.07±2.09)%, (31.28±4.65)%, (22.91±1.68)% and (20.94±3.20)%. [Conclusion] The apparent inhibitory effects of flavonoids on platelet aggregation are potentially associated with their intrinsic structures. The 7-OH group of A ring and 3-OH group of C ring were important for its antiplatelet function. |
| Key words: flavonoids platelet aggregation structure function |
