水通道蛋白-1在急性肺损伤中的表达及意义

Expression of AQP-1 in acute lung injury induced by lipopolysacharide in rats

关键词:  急性肺损伤  脂多糖  水通道蛋白-1

Key words:  acute lung injury  lipopolysaccharide  aquaporin-1

摘要:

[目的] 探讨水通道蛋白1（AQP-1）在脂多糖（LPS）所致的急性肺损伤(ALI)中的作用。[方法] 根据是否腹腔注射脂多糖（LPS）将实验动物分为脂多糖组（LPS组）及生理盐水对照组（Con组），对比两组动物组织病理学改变、动脉血气、肺湿/干重比及AQP-1表达的差异。[结果] 光镜下见LPS组肺组织水肿，大量炎性细胞浸润；LPS组PO2（55.07±17.02）mmHg明显低于Con组（97.35±9.37）mmHg，差异有显著性意义，P<0.05；LPS组肺湿／干重比（4.93±0.16）明显高于Con组（4.11±0.69），差异有显著性意义，P<0.05；免疫组化染色显示：LPS组的AQP-1蛋白表达减弱，荧光实时定量PCR证实LPS组的AQP-1 mRNA表达较对照组明显下降（P<0.01）。[结论] AQP-1参与了急性肺损伤的形成。

Abstract:

[Objective] To investigate the expression and effect of AQP-1 in acute lung injury (ALI) induced by lipopolysaccharide. [Methods] Twenty Sprague-Dawley rats were randomly divided into LPS group treated with LPS injection and normal control group injected with saline (Control group). Arterial oxygen tension (PaO2) and wet/dry mass ratio (W/D) were tested at 6th hour after the injection. The expressions of AQP-1 protein in the lungs of rats were detected by immunohistochemistry and the expressions of AQP-1 mRNA were measured by a real-time fluorescence PCR. [Results] The arterial oxygen tension (PaO2) in LPS group was obviously lower than that in control group (55.07±17.02) mmHg vs (97.35±9.37) mmHg (P<0.05). The wet/dry mass ratio (W/D) in LPS group was obviously higher than that in control group (4.93±0.16 vs 4.11±0.69, P<0.05). The immunostaining showed a significant decrease in the expression of AQP-1 and a real time RT-PCR confirmed that the levels of AQP-1 mRNA were significantly reduced in LPS group (P<0.01) at 6 h after LPS injection. [Conclusion] AQP-1 has an great effect in acute lung injury (ALI).