小补心汤总黄酮对慢性应激小鼠脑内单胺递质水平的影响

Effect of the total flavonoids extracted from Xiaobuxin-Tang on monoamine neurotransmitter in chronically stressed mice

关键词:  小补心汤  总黄酮  单胺递质  5-HT  应激

Key words:  XBXT  total flavonoids  monoamine neurotransmitter  5-HT  stress

摘要:

［目的］探讨小补心汤总黄酮（代号：XBXT-2）对慢性应激小鼠脑组织单胺递质及其代谢产物含量的影响。［方法］建立小鼠慢性应激模型，XBXT-2（25、75 mg/kg）或丙咪嗪（10 mg/kg）每日单次灌胃给药，连续给药21 d。用高效液相加电化学检测（HPLC-ECD）的方法检测全脑组织去甲肾上腺素（NE）、多巴胺（DA）、其代谢产物3，4-二羟基苯乙酸（DOPAC）、高香草酸（HAV）、五羟色胺（5-HT）及其代谢产物五羟吲哚乙酸（5-HIAA）的含量。［结果］HPLC-ECD结果表明，慢性应激小鼠脑组织DA、DOPAC、HAV、5-HT、5-HIAA含量均明显降低（P<0.05），XBXT-2（25、75 mg/kg）长期给药可显著增加5-HT的含量，分别为（365.0±215.0）、（313.5±203.0）ng/g 脑组织，P<0.05；及其代谢产物5-HIAA的含量，分别为（352.4±162.7）ng/g 脑组织，P<0.01和（295.4±116.6）ng/g 脑组织，P<0.05。且XBXT-2（75 mg/kg）具有促进DA代谢转化为HAV的作用（P<0.05）。［结论］XBXT-2能增强中枢5-HT系统的功能，这可能是其抗抑郁作用的神经生化机制之一。

Abstract:

［Objective］To investigate the effect of the total flaconoids extracted from Xiaobuxin-Tang (XBXT-2) on monoamine neurotransmitter in chronically stressed mice.［Methods］The chronically stressed mouse model was made, and then XBXT-2 (25,75 mg/kg) and IMI (10 mg/kg) were administrated intragastrically once a day for 21 days.High-performance liquid chromatography with electrochemical detection (HPLC-ECD) was applied to measure the levels of norepinephrine (NE),dopamine (DA),its metabolite homovanillic acid (HVA) and 3,4-hihydroxyphenylacetic acid (DOPAC),serotonin (5-HT) and its metabolite 5-hydroxyindole-3-acetic acid (5-HIAA).［Results］HPLC-ECD results showed that the levels of 5-HT,5-HIAA,DA,HVA and DOPAC were significantly decreased in chronically stressed mice (P<0.05),and concomitant administration of XBXT-2 (25,75 mg/kg) significantly increased the levels of 5-HT (365.0 ±215.0),(313.5±203.0)ng/g brain tissue,P<0.05,and its metabolite 5-HIAA (352.4±162.7)ng/g brain tissue,P<0.01;(295.4±116.6)ng/g brain tissue,P<0.05.Additionally,XBXT-2 (75 mg/kg) could increase the turnover of DA to HAV(P<0.05).［Conclusion］XBXT-2 exerted significant serotonergic activation in CNS of chronically stressed mice,which may be one of the primary neurochemistry mechanisms underlying the antidepressant effect of XBXT-2.