烟酰胺磷酸核糖转移酶在大鼠涎腺的表达及定位

Expression and localization of nicotinamide phosphoribosyl transferase in salivary glands of rat

关键词:  烟酰胺磷酸核糖转移酶  腮腺  颌下腺  大舌下腺

Key words:  nampt  parotid gland  submandibular gland  major sublingual gland

摘要:

［目的］研究烟酰胺磷酸核糖转移酶(nicotinamide phosphoribosyl transferase,Nampt)在大鼠涎腺的表达和分布。［方法］应用半定量逆转录聚合酶链法（RT-PCR）和免疫印迹法检测大鼠正常腮腺、颌下腺和大舌下腺组织Nampt mRNA及蛋白质的表达。采用免疫组织化学法检测大鼠腮腺、颌下腺和大舌下腺组织Nampt的分布。［结果］大鼠腮腺、颌下腺和大舌下腺组织均有Nampt的mRNA及蛋白质表达，RT-PCR结果显示其表达相对光密度值分别为：腮腺82.04%，颌下腺50.89%，大舌下腺30.53%（与actin比较，P<0.05）；Western Blot结果显示其表达相对光密度值分别为：腮腺77.34%，颌下腺47.61%，大舌下腺31.78%（与actin比较，P<0.05）。Nampt均主要分布于浆液性腺泡细胞的胞浆和胞核，以及闰管和纹状管的胞核中。［结论］大鼠腮腺、颌下腺和大舌下腺均存在Nampt的mRNA和蛋白质表达，为深入研究Nampt在涎腺生理及病理状态下的功能意义提供初步实验依据。

Abstract:

［Objective］To investigate the expression and distribution of nicotinamide phosphoribosyl transferase (Nampt) in rat salivary glands.［Methods］The expressions of Nampt mRNA and protein were investigated by reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot in the parotid,submandibular and major sublingual glands of rat. Immunohistochemical assay was applied to detect the distribution of Nampt in these glands too.［Results］The results of RT-PCR showed that the optical density of the expression of Nampt mRNA was 82.04%,50.89% and 30.53% respectively in the parotid,submandibular and major sublingual glands of rat (P<0.05,compared with actin).The results of Western blot showed that the optical density of the expression of Nampt protein was 77.34%,47.61% and 31.78%,respectively in these glands (P<0.05,compared with actin).Nampt protein was widely distributed in them, mainly localized in the cytoplasm and nucleus of both serous acinar cells and ductal cells.［Conclusions］Nampt mRNA and protein are expressed in the parotid,submandibular and major sublingual glands of rat,and may be involved in underlying mechanisms of physiology and patho-physiology in the salivary glands.