TopoⅡ、P170、GST-pi蛋白在胃癌中表达与临床病理及预后的关系

Expression of TopoⅡ,P170 and GST-pi in gastric carcinoma and their correlations with clinical prognosis

关键词:  耐药基因P-糖蛋白（P170）  DNA拓扑异构酶Ⅱ（TopoⅡ）  谷胱苷肽-S转移酶（GST-pi）  胃癌

Key words:  p-170 glycoprotein (P170)  DNA topoisomerases,type II (TopoⅡ)  glutathione S transferase pi (GST-pi)  gastric neoplasms

摘要:

［目的］探讨胃癌中P-糖蛋白（P170）、DNA拓扑异构酶Ⅱ（TopoⅡ）和谷胱苷肽-S转移酶（GST-pi）的表达和胃癌的临床病理特征及其预后的关系及意义。［方法］免疫组化检测160例胃癌组织中的P170、TopoⅡ及GST-pi的表达，收集临床病理资料，随访患者的生存时间。分析各因子表达、临床病理资料和预后的关系。［结果］P170和GST-pi的表达呈正相关（P<0.01），P170表达与肿瘤直径、淋巴结转移、远处转移及TNM分期呈正相关（P<0.05）。GST-pi表达与远处转移及TNM分期呈正相关（P<0.05）。TopoⅡ的表达与侵袭深度、远处转移及TNM分期呈正相关（P<0.05）。单因素分析发现患者年龄、肿瘤直径、肿瘤部位、病理分级、侵袭深度、淋巴转移、远处转移、TNM分期、TopoⅡ、P170、GST-pi均与预后有关（P<0.05）。Cox风险模型多因素分析发现肿瘤部位、病理分级、侵袭深度、淋巴转移、GST-pi是影响胃癌预后的独立因素（P<0.05）。［结论］TopoⅡ、P170、GST-pi均与胃癌的某些临床病理特征呈一定的相关性，肿瘤部位、病理分级、侵袭深度、淋巴转移、GST-pi是影响胃癌预后的独立因素。

Abstract:

［Objective］To evaluate the expression of P-glycoprotein (P170),Topoisomerase Ⅱ (TopoⅡ) and glutathione S-transferase π (GST-pi) in gastric carcinoma,their possible relationship with clinicopathologic parameters of tumors,and their prognostic significance.［Methods］ The expression of P170,TopoⅡ and GST-pi in 160 patients with gastric carcinoma was detected by immunohistochemistry and the patients were followed up for more than three years.The relationship between the expression of P170,TopoⅡ,GST-pi and the clinicopathologic parameters were analyzed.The relationship between these parameters and prognosis of patients were evaluated.［Results］There was a positive correlation between the expression of P170 and GST-pi.The intensity of P170 was positively related to the diameter of tumor,lymphatic metastasis,distant metastasis and TNM grade (P<0.05).The expression of GST-pi was associated to the pathological grade,distant metastasis and TNM grade (P<0.05).The expression strength of TopoⅡwas positively related to the pathological grade,lymphatic metastasis,depth of invasion and TNM grade (P<0.05).In univariate analysis,age,tumor diameter,tumor site,histological type,depth infiltration,lymphatic metastasis,distance metastasis,TNM grade and the expression of Topo Ⅱ,P170,GST-pi were related to the prognosis of gastric cancer (P<0.05).Multivariate Cox model analysis showed that the tumor site,histological type,depth infiltration,lymphatic metastasis and GST-pi were the independent prognostic factors for patients (P<0.05).［Conclusion］The expression of TopoⅡ,P170,GST-pi are related to some of the histopathological features,and some of these factors can be regarded as prognostic factors in gastric carcinoma.