抗菌肽对高龋患者口内变异链球菌生长的影响

Effect of antibacterial peptide on Streptococcus mutans isolated from caries-active individuals

关键词:  抗菌肽  龋病  变异链球菌

Key words:  antibacterial peptide  caries  streptococcus mutans

摘要:

［目的］研究人工合成抗菌肽KSL对高龋患者口内变异链球菌的生长抑制情况，为KSL的临床实用奠定实验基础。［方法］依据抗菌肽KSL的氨基酸序列人工合成抗菌肽KSL，采用标准培养法及生化鉴定法，分离鉴定高龋患者口内变异链球菌，测定KSL对该菌株的生长抑制情况。［结果］成功分离鉴定变异链球菌3株，KSL对临床分离变异链球菌的MIC为0.0625 mg/mL，MBC为1.0000 mg/mL，KSL对变异链球菌杀菌曲线表明应用30 min后其对变异链球菌有明显的杀菌效果。［结论］抗菌肽KSL对高龋患者口内变异链球菌有着明显的抑菌杀菌效果。

Abstract:

［Objective］To study the effect of synthesized antibacterial peptide KSL on inhibiting the Streptococcus mutans isolated from caries-active individuals for its application in clinical.［Methods］The antibacterial peptide KSL was synthesized according to its amino acid sequence. The standard cultivation and biochemistry methods was applied to isolate and identity the Streptococcus mutans from caries-active and the KSL’s effects on inhibiting the Streptococcus mutans growth was detected. ［Results］Three strains of Streptococcus mutans were isolated successfully,the MIC of KSL on the clinical strain was 0.0625 mg/mL,and the MBC was 1.0000 mg/mL,the killing curve of Streptococcus mutans indicated that 30 minutes after KSL administration had the evidently bactericidal results. ［Conclusion］Antibacterial peptide KSL have the evidently inhibitory and bactericidal effects on Streptococcus mutans isolated from caries-active individuals.