食管癌S100A4蛋白的表达特点

Expression characteristics of S100A4 in esophageal carcinoma

关键词:  S100A4  食管癌  蛋白表达  免疫组织化学染色

Key words:  S100A4  esophageal carcinoma  protein expression  immunohistochemistry stain

摘要:

［目的］探讨食管癌转移相关蛋白S100A4的表达特点及意义。［方法］用石蜡组织微阵列免疫组织化学ABC方法，检测S100A4在83例食管癌组织、38例癌前病变、9例非癌粘膜的表达和分布特点。［结果］S100A4表达率在食管癌、癌前病变与癌旁组织分别为59.0%，26.3%与22.2%，食管癌组显著高于癌旁正常组及癌前病变组（P<0.05），但后两组间差异无显著性意义（P>0.05）。腺癌与鳞癌该蛋白表达的阳性率分别为80%与54.4%，两者比较差异有显著性意义（P<0.05）。S100A4的表达率在女性患者与男性患者分别为84.6%与54.3%，二者差异具有显著性意义（P<0.05）；在淋巴结转移组与无淋巴结转移组分别为70.5%与46.2%，二者差异具有显著性意义（P<0.05）；但与患者年龄、肿瘤大小、分化程度无显著相关性（P>0.05）。［结论］S100A4蛋白表达是食管癌发生、发展、演进过程中的分子事件，其表达与食管癌进展密切相关。

Abstract:

［Objective］To profile expression of S100A4 during stepwise esophageal carcinogenesis and to analyze the relationship to the series of pathological factors.［Methods］Paraffin embedded tissue array with immunohistochemistry methods were adopted to exam the expression pattern of S100A4 in 83 esophageal carcinomas (EC), 38 precancerous lesions and 9 noncancerous esophageal tissues, and the correlations between S100A4 expression and EC invasiveness and metastasis were analyzed.［Results］The expression rates of S100A4 in EC, premalignant and noncancerous tissues twere, 59.0% 26.3% and 22.2%, the expression rate of EC was significantly higher than those of noncancerous and precancerous (P<0.05), while statistical differences was found between the noncancerous and precancerous group (P>0.05). The expression rate of adenocarcinoma (80.0%) was significantly higher than that of squamous cell carcinoma (54.4%), and the rate of female (84.6%) was significantly higher than that of the male (54.3%)(P<0.05). The protein expression was not statistically related to patient age and tumor differentiation and size (P>0.05). The expression rate of lymph node metastatic group(70.5%) was significantly higher than that of non lymph node metastaticgroup(46.2%),(P<0.05).［Conclusions］The expression of S100A4 is an important molecular event in esophageal carcinogenesis, and progression.