| 摘要: |
| ［目的］探讨肿瘤坏死因子-α（TNF-α）对滋养细胞增殖、凋亡及绒毛膜促性腺激素(HCG)分泌调节的影响。［方法］建立人早期妊娠滋养细胞体外培养体系,检测不同浓度TNF-α作用下滋养细胞增值状态及HCG的浓度。［结果］TNF-α在低浓度时促进滋养细胞的增殖活性,在高浓度时则抑制其增殖活性； TNF-α浓度为10 ng/mL、100 ng/mL 可使滋养细胞凋亡，其凋亡率随TNF-α浓度升高而增加（P<0.05）；在0~100 ng/mL浓度TNF-α范围内，滋养细胞分泌HCG水平随TNF-a浓度的增加而升高。［结论］TNF-α可影响滋养细胞增殖与凋亡；且具有促进滋养细胞分泌HCG的功能。 |
| 关键词: 肿瘤坏死因子-α 滋养细胞 绒毛膜促性腺激素 细胞凋亡 |
| DOI：10.11724/jdmu.2010.03.02 |
| 分类号:R692.6 |
| 基金项目: |
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| Effect of TNF-α on proliferation and HCG production of human trophoblasts cell from first trimester placentae of human origin |
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ZHANG Xin-yu1, WANG Mi2, YIN Bing3
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1.Department of Gynaecologics and Obstetrics, the First Affilitate Hospital of Dalian Medical University, Dalian 116011, China;2.The Maternal and Child Care Center of Qingdao, Qingdao 266000, China;3.Department of Nursing, Dalian Medical University, Dalian 116044, China
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| Abstract: |
| ［Objective］To investigate the potential role of tomors necrosis factor-α (TNF-α) in inducing proliferation, apoptosis of human trophoblasts and its secretion of HCG.［Methods］Detecting the early apoptosis rate and the level of HCG secretion in cultured human trophoblasts.［Result］The TNF-α in low concentration could stimulate trophoblasts toprolife
rate, while in the high concentration could inhibit it.TNF-α of 10 ng/mL、100 ng/mL induced the apoptosis of trophoblasts with dose dependent(P<0.05). Within TGF-α concentration of 0~100 ng/mL, the trophoblasts secrete HCG with dose dependent(P<0.05).［Conclusion］TNF-α influence human trophoblasts proliferation and apoptosis and enhanced its HCG secretion. |
| Key words: TNF-α human trophoblast HCG apoptosis |
