| 摘要: |
| ［目的］探讨HCV核心抗原在HCV感染诊断和治疗中的作用。［方法］对173例HCV感染患者血清和82例健康对照者血清，使用ELISA法分别检测抗-HCV、总的HCV核心抗原，使用荧光定量RT-PCR检测HCV RNA。［结果］173例HCV感染者抗-HCV均为阳性。HCV RNA阳性率为49.7%（86/173）；HCV核心抗原阳性率为36.4%（63/173）。二者差异有统计学意义（P<0.05）。86例HCV RNA阳性标本中，HCV核心抗原阳性60例（69.8%）；HCV核心抗原阴性26例（30.2%）。82例健康对照者抗-HCV、HCV核心抗原、HCV RNA均为阴性。［结论］在没有HCV RNA检测资格的实验室，HCV核心抗原检测可作为病毒存在和复制的一个指标，作为抗-HCV检测的补充试验。 |
| 关键词: 丙型肝炎病毒 核心抗原 抗体 RNA |
| DOI：10.11724/jdmu.2010.02.26 |
| 分类号:R446 |
| 基金项目: |
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| Clinical significance of testing hepatitis C virus core antigen |
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ZHU Hong, ZHANG Tao, FAN Cun-lin, XIAO Xiao-guang, Zhang Feng-hua
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Department of Clinical Laboratory,the First Affiliated Hospital of DaLian Medical University,Dalian 116011,China
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| Abstract: |
| ［Objective］To investigate the significance of serum total HCV core antigen (HCV-cAg) in the HCV infection and treatment.［Methods］One hundred and seventy-three serum specimens of HCV infection patients were examined for HCV cAg and anti-HCV antibody with ELISA,for HCV RNA with real-time RT-PCR.［Results］In 173 anti-HCV positive specimens,the positive rate of HCV RNA was 49.7% (86/173) and the positive rate of HCV-cAg was 36.4% (63/173).The positive rate of HCV RNA was higher than that of HCV-cAg (P<0.05).In 86 HCV RNA positive specimens,the positive rate of HCV-cAg was 69.8% (60/86),the negative rate was 30.2% (26/86).The control was negative.［Conclusions］In laboratory where it is impossible for HCV RNA measurement by using RT-PCR,HCV-cAg assay in serum will be very useful for the clinical diagnosis of hepatitis C and the detection of HCV. |
| Key words: Hepatitis C virus core antigen antibody RNA |
