纳米银对流感病毒H3N2灭活作用的探讨

Inactivation of nanosilver on influenza virus H3N2

关键词:  纳米银  流感病毒H3N2  灭活作用

Key words:  nanosilver  influenza virusv H3N2  inactivation

摘要:

［目的］探讨纳米银(Nanosilver,NaAg)对流感病毒H3N2的灭活作用。［方法］采用血球凝集试验、鸡胚培养法、MTT分析法、血球吸附试验探讨NaAg对流感病毒H3N2的灭活作用。利用透射电镜、流式细胞仪检测病毒诱导细胞的凋亡。［结果］NaAg/ H3N2组血球凝集试验效价为1∶2，鸡胚尿囊液血凝效价低于1∶2，流感病毒H3N2对照组血球凝集试验效价、鸡胚尿囊液血凝效价均为1∶1024，二者比较差异具有显著性意义（P<0.001）；NaAg溶液在狗肾MDCK细胞上最大无毒浓度为25 μg/mL；流感病毒H3N2在 MDCK细胞上半数感染浓度（TCID50）为10-3.5/0.1 mL。等体积的50 μg/mL NaAg与40 TCID50 流感病毒H3N2溶液室温充分混和作用2 h后感染MDCK细胞，细胞的存活率为（98.16±2.67）%，而20 TCID50 流感病毒H3N2感染MDCK细胞，细胞的存活率为（35.21±1.45）%，二者比较差异具有非常显著性意义（P<0.001）。25 μg/mLNaAg溶液有效抑制了20 TCID50 流感病毒 H3N2诱导MDCK的细胞凋亡。［结论］纳米银对流感病毒H3N2具有直接灭活作用。

Abstract:

［Objective］To study the inactivation of nanosilver (NaAg) on influenza virus H3N2.［Methods］The hemagglutination test, the cultivation in the chick embryo test, the MTT test and the haemocyanin absorption test were used to determine the inactivation of NaAg on influenza virus H3N2. The TEM and the flow cytometer were adopted to detected the apoptosis of cells induced by virus.［Results］In NaAg/H3N2 group, the titer of hemagglutination and that of allantoic fluid of chick embryo was 1∶2 and lower than 1∶2, but in the control group of influenza virus, the titers of both were 1∶1024 (P<0.001). The maximal atoxic concentration(TD0) of NaAg on MDCK cell was 25 μg/mL, the TCID50 of influenza virus H3N2 on MDCK cell was 10-3.5/0.1 mL. The survival rate of MDCK cells was (98.16±2.67)% after the 50 μg/mL NaAg solution mixed with 40 TCID50 of influenza virus H3N2 in 2 hours, and the survival rate of MDCK cells was (35.21±1.45)% in the influenza virus H3N2 control group with 20 TCID50 (P<0.001). NaAg of 25 μg/mL inhibited effectively the apoptosis of MDCK cells induced by 20 TCID50 influenza virus H3N2.［Conclusion］The nanosilver could inactivatInfluenza virus H3N2.