超声时间与药材粒度对超声法提取槐花米中芦丁的影响

Effect of time and particle size on yield of Rutin from Sophora japonica L. by ultrasonic-asisted extraction

关键词:  芦丁  超声法  提取

Key words:  Rutin  ultrasonic wave  extraction

摘要:

［目的］ 研究超声时间及药材粉碎粒度对超声法提取芦丁产率的影响，从而提高芦丁的提取效率。［方法］ 将不同粉碎粒度的槐花米粉末溶于碱液，超声提取不同时间后，检测不同时间点及不同粉碎粒度药材的芦丁产率，探索超声时间及粉碎粒度对芦丁提取产率的影响。［结果］ 粉碎至不同粒度的槐花米粉末，超声提取后所得芦丁产率在一定时间范围内均与超声时间正相关，而后呈平台趋势，在本实验条件下，采用3号筛的槐花米粉末超声5 min后即达到较高提取产率16.46%。［结论］ 药材粉碎粒度及超声时间对芦丁提取效率有着明显影响，超声辅助提取法较传统的碱提酸沉法具有流程短、效率高及成本低等优点。

Abstract:

［Objective］ To study the extraction time and the particle size of crude material which may have effects on the yield of rutin from Sophora japonica L. by ultrasonic-assisted extraction (UAE). ［Methods］ The different particle sizes of S. japonica L. were extracted with alkali-water by UAE after pulverization, and the contents of rutin were detected at different times. ［Results］ The results showed that there was a positive correlation between the particle size of crude materials and the product yield of rutin within a certain time. After pulverized to the size of 3 sieves meshes and extracted for 5 minutes, the yield of rutin from Sophora japonica L. reached 16.4%. ［Conclusions］ The time and the particle size have significantly effect on the yield of rutin from Sophora japonica L. by UAE. In comparison with the acid-base extraction, the UAE has advantage of short process, high-efficiency and low-cost.