4种不同抗菌药物与磷霉素联用对耐甲氧西林葡萄球菌体外抗菌活性的影响

Analysis of in vitro antibacterial activity to fosfomycin in combination with different antimicrobial agents respectively to Methicillin-resistant Staphylococcus

关键词:  磷霉素  耐甲氧西林葡萄球菌  最小抑菌浓度

Key words:  fosfomycin  Methicillin-resistant Staphylococcus (MRS)  MIC

摘要:

［目的］ 研究头孢唑啉、氧氟沙星、红霉素、阿米卡星4种抗菌药物分别与磷霉素联合对临床分离的耐甲氧西林葡萄球菌（MRS）菌株的体外抗菌活性。［方法］ 将磷霉素等5种抗菌药物各自单独以及4种抗菌药物分别与磷霉素联合作用于153株MRS菌株，测定其最小抑菌浓度（MIC），计算联合药敏指数（FIC），绘制杀菌曲线，电镜扫描观察细菌形态，评价4种抗菌药物分别与磷霉素联合应用的体外抗菌活性。［结果］ 头孢唑啉、氧氟沙星、红霉素、阿米卡星分别与磷霉素在体外联合应用后，其联合用药的MIC值均比单独应用时下降，联合药敏指数均≤2；联合应用对MRS杀菌速度加快，杀菌作用增强。［结论］ 头孢唑啉、红霉素、阿米卡星、氧氟沙星分别与磷霉素联合均具有协同抗菌作用，能降低对MRS的MIC。

Abstract:

［Objective］ To study the effect of antibacterial activity in vitro for Methicillin-resistant Staphylococcus (MRS) on fostomycin, when combined with β-lactam,aminoglycoside, macrolide or fluroquinolone respectively. ［Methods］ One hundred and fifty-three strains of MRS were treated by 5 antibacterial drugs including fosfomycin and by combination with fostomycin and other 4 antibacterial drugs respectively. In vitro antibacterial activities were evaluated by testing MIC, calculating FIC index, drawing time killing curve and observing morphological change of bacterial through scanning electron microscope. ［Results］ When cefazolin, erythromycin, amikacin,ofloxacin were used in combination with fostomycin respectively, the MIC of drug combination declined if compared with used alone and FIC index ≤2. The antimicrobial speeds of combination were increased, while bactericidal effect was strengthened. ［Conclusion］ Cefazolin, erythromycin, amikacin, ofloxacin in combination with fostomycin respectively can reduce the drug MIC.