姜黄素协同β-榄香烯诱导涎腺腺样囊性癌细胞株SACC-83凋亡的研究

Study on the apoptosis of adenoid cystic carcinoma cell Line SACC-83 induced by curcumin and β-elemene

关键词:  涎腺腺样囊性癌细胞株SACC-83  细胞周期  姜黄素  β-榄香烯

Key words:  salivary adenoid cystic carcinoma  SACC-83 cell line  cell cycle  curcumin  β-elemene

摘要:

［目的］ 研究姜黄素协同β-榄香烯诱导体外培养涎腺腺样囊性癌细胞株SACC-83凋亡的情况。 ［方法］应用MTT测定法、流式细胞术和透射电镜观察等方法观察姜黄素协同β-榄香烯（浓度比1∶1）诱导SACC-83细胞凋亡及其与G₂/m期阻滞关系。 ［结果］（1）应用MTT测定法，姜黄素协同β-榄香烯（浓度比1∶1）作用SACC-83细胞24 h后，其抑制率有明显协同效应。 （2）流式细胞术分析，10 μg/mL 姜黄素与10 μg/mL β-榄香烯联合作用SACC-83细胞24 h后诱导SACC-83细胞发生凋亡，并将细胞周期阻滞于G₂/m期。 （3）透射电镜观察，协同用药作用SACC-83细胞24 h后，出现明显细胞凋亡特征。［结论］ 姜黄素和β-榄香烯（浓度比1∶1）对SACC-83细胞的抑制有协同作用，并诱导其产生凋亡，使其细胞周期阻滞于G₂/m期。

Abstract:

［Objective］ To study the apoptosis of salivary adenoid cystic carcinoma cell line SACC-83 induced by curcumin and β-elemene.［Methods］ Curcumin and β-elemene (concentration 1:1) induced arrest of cell G₂/m phase and apoptosis of SACC-83 cells were determined, which were checked by MTT color assay and observed with flow cytometry and electronmicroscope.［Results］ Using MTT test, the synergistic effect of curcumin and β-elemene could inhibit SACC-83 cells effectively after 24 hours, which did SACC-83 cell growth in a dose-dependent manner. SACC-83 cells were induced to apoptosis in 24 hours by 10 μg/mL curcumin and 10 μg/mL β-elemene. SACC-83 cells were blocked during G₂/m phase. Cells undergoing apoptosis exhibited the following typical features: an apparent apoptotic peak during section of cell cycle with flow cytometry. 10 μg/mL curcumin and 10 μg/mL β-elemene (with 24 hours) inhibited SACC-83 cells. The trypical uLtra-structral changes of apoptosis observed by electron-microscope was chromatin's cling to periphery of the shrunken nuclear.［Conclusions］ The synergistic effect of curcumin and β-elemene (concentration 1:1) could inhibit SACC-83 cells and induce apoptosis.Arrest of G₂/m phase might cause apoptosis of SACC-83 cells.