全反式维甲酸对慢性阻塞性肺疾患大鼠肺中性粒细胞趋化因子MIP-2分泌的影响

Effects of All-trans retinoic acid on production of MIP-2 of lungs in rats with chronic obstructive pulmonary diseases

关键词:  慢性阻塞性肺疾患  维甲酸  MIP-2  髓过氧化物酶

Key words:  COPD  ATRA  MIP-2  MPO

摘要:

［目的］研究全反式维甲酸对慢性阻塞性肺疾患大鼠肺中性粒细胞功能的影响及机制。［方法］SD大鼠24只，随机分为模型组、维甲酸组和对照组，每组8只。每日熏烟1 h共28 d，在第0天和第14天气管内滴脂多糖（LPS）复制COPD动物模型，维甲酸组经食管灌入维甲酸，模型组不做治疗，正常大鼠为对照组。第28天杀死大鼠，取肺组织用于形态学检查或并制备肺组织匀浆，用ELISA法检测肺组织匀浆中MIP-2的变化，用比色法检测髓过氧化物酶（MPO）的变化。［结果］应用维甲酸的大鼠平均肺泡直径变小，平均肺泡数增多；维甲酸组与模型组肺组织匀浆MIP-2含量分别为（16.62±6.03）pg/mL与（41.43±7.36）pg/mL，肺组织匀浆过氧化物酶髓活性分别为（9.13±2.15）U/g与（12.2±1.72）U/g。［结论］通过抑制MIP-2分泌抑制中性粒细胞功能可能是维甲酸防治慢性阻塞性肺疾患发生的机制之一。

Abstract:

［Objective］ To investigate the effect of all-trans retinoic acid on production of MIP-2 of lungs in rats with chronic obstructive pulmonary diseases (COPD). ［Methods］ Twenty two rats were randomly divided into model, retinoid acid treatment or control groups (n=8). The rats were inhaled with tobacco smoke every day for 28 days and instilled intratracheally with E.coli lipopolysaccharide (lipopolysaccharide, LPS) on day 1st and 14 th with all-trans retinoic acid gavages(ATRA group) or not treated (model group). Normal rats were used to control group. On day 28 th all of rats were sacrificed. Their lungs were used to morphological observation changes and tissue homogenate. The content of MIP-2 was measured with ELISA.Activity of Myeloperoxidase (MPO) was measured with colorimetric method.［Results］The diameter of alveoli of the rats treated with all-trans retinoic acid was decreased, the number of alveoli each field of microscope was increased. In ATRA and COPD modle groups, the MIP contents were (16.62±6.03)pg/mL and (41.43±7.36)pg/mL, the MPO activities were (9.13±2.15)U/g and (12.2±1.72)U/g.［Conclusion］Inhibiting the production and function of neutrophils may be one of mechanism by which all-trans retinoic acid treat COPD.