PGC-1α基因Gly482Ser多态性与2型糖尿病发病的相关性研究

Association of Peroxisome proliferator activated receptor-γ coactivator-1 α (PGC-1α) Gly482Ser polymorphism with type 2 diabetes mellitus in Chinese women

关键词:  2型糖尿病  过氧化物酶体增殖物激活受体γ辅激活因子-1α  遗传/多态性

Key words:  diabetes mellitus/type 2  PGC-1α  polymorphism/genetics

摘要:

［目的］ 研究过氧化物酶体增殖物激活受体γ共活化物-1α（PGC-1α）Gly482Ser基因多态性与2型糖尿病发病的相关性。［方法］ 选取大连居住的汉族人2型糖尿病病人（DM）140例和非糖尿病对照（NC）88人；用聚合酶链反应（PCR）-限制性内切酶技术检测PGC-1α Gly482Ser基因型。［结果］ DM组Ser/Ser基因型分与NC组比较差异有显著性意义（χ²=10.969，P=0.004），按性别分层后男性DM组与NC组比较差异无显著性意义（χ²=3.601，P=0.165），而女性DM组与NC组比较差异有显著性意义（χ²=11.02，P=0.004）。在NC组和DM组，随Ser等危基因增加，空腹C肽和2 h C肽呈逐渐降低趋势，但只有Ser/Ser与Gly/Gly两基因型之间相比差异有显著性意义（P<0.05）。在NC组，各基因型之间的胰岛素抵抗之间比较差异无没有显著性意义（P>0.05）。［结论］ PGC-1 Gly482Ser多态性可能与女性2型糖尿病的发病相关，Ser/Ser型携带者C肽水平更低。

Abstract:

［Objective］ To study if PGC-1α Gly482Ser polymorphism is related to type 2 diabetes mellitus in Chinese. ［Methods］ One hundred and forty type 2 diabetes (DM) and 98 non-diabetic controls (NC) were selected. Genotyping of PGC-1α Gly482Ser was determined with PCR amplification, followed by MspI digestion. ［Results］ PGC-1α Ser/Ser genotype frequency in DM was significantly higher than that in NC (29% vs 12.5%, χ²=10.969, P=0.004). Upon stratification with sex Ser/Ser genotype was significantly higher in DM women than NC women (χ²=11.02, P=0.004), while it was not so in men. Both in NC and DM, fasting C-peptide and 2 hour postprandial C-peptide levels were lower in subjects with Ser/Ser genotype than in subjects with Gly/Gly genotype (P<0.05). There was no significance for insulin resistance among different genotypes both in DM and NC groups (P<0.05). ［Conclusion］ The PGC-1α Gly482Ser polymorphism might be related to the development of type 2 diabetes in Chinese women. Ser/Ser seems to be associated with lower endogenous insulin secretion.