脊索瘤细胞系CM-319中放化疗抵抗相关基因的表达及临床意义

Expression of resistance genes against chemotherapy and radiotherapy and the clinical significance in chordoma cell line CM-319

关键词:  缺氧诱导因子1α  多药耐药基因1  多药耐药相关蛋白1  脊索瘤

Key words:  hypoxia inducible factor-1α  multidrug resistance gene 1  multidrug resistance-associated proteins 1  chordoma

摘要:

［目的］ 探讨多药耐药相关基因如缺氧诱导因子1α（HIF-1α）、多药耐药基因（MDR1）及多药耐药相关蛋白（MRP1）在脊索瘤细胞系CM-319中的表达及临床意义。［方法］ 用RT-PCR检测HIF-1α、MDR1、MRP1基因表达，间接免疫荧光及免疫细胞化学Envision法检测HIF-1α、MRP1蛋白在CM-319中的表达情况，并分析其与放化疗抵抗的关系。［结果］ 常氧条件下，在脊索瘤细胞系中能检测到HIF-1α及MRP1 mRNA的表达，但未检测到MDR1 mRNA的表达（P<0.01），间接免疫荧光及免疫细胞化学可以检测到HIF-1α表达在细胞浆及核中，而MRP1主要表达在细胞浆中。［结论］ HIF-1α与MRP1的过度表达在脊索瘤的放化疗抵抗中可能具有重要作用，而MDR1表达未被检测到，提示脊索瘤的放化疗抵抗主要与HIF-1α和MRP1相关。

Abstract:

［Objective］ To investigate the expression of some genes related to resistance to chemotherapy and radiotherapy, such as HIF-1α, MRP1 and MDR1, and their clinical significance in chordoma cell line CM-319. ［Methods］ The expression of HIF-1α, MDR1 and MRP1 was detected with reverse transcription polymerase chain reaction (RT-PCR) and indirect immunofluorescence staining and immunocytochemical technique (ChemMate^TM Envision). The relationship between HIF-1α, MRP1 and resistance to chemotherapy and radiotherapy was statistically analyzed. ［Results］ The positive expression of HIF-1α and MRP1 was detected in CM-319 in normal Oxygen conditions, while MDR1 was not detected by RT-PCR( P < 0.01 ). The positive expression of HIF-1α protein was detected in cytoplasm and nucleus, and MRP1 mostly in cytoplasm with indirect immunofluorescence staining and immunocytochemical technique. ［Conclusions］ The overexpression of HIF-1α, MRP1 plays an important role in the resistance to chemotherapy and radiotherapy of chordoma cell line. And the expression of MDR1 was not detected. It may be a signature that the resistance to chemotherapy and radiotherapy was related with HIF-1α, MRP1 in chordoma cell line.